Mutagenic Effect of Gamma Irradiation on Phenotype and Enzyme Activities in Aspergillus niger | ||||
| Egyptian Journal of Botany | ||||
| Article 10, Volume 56, Issue 2, February 2016, Page 507-526 PDF (454.12 K) | ||||
| DOI: 10.21608/ejbo.2017.1148 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Esmat E. Aly1; Neveen M. Khalil* 1; G. Abd- El-Hamid2; M.A. Abo-El-Soued2; Heba S. Mostafa2 | ||||
| 1Botany and Microbiology Department, Faculty of Science, Cairo University | ||||
| 2Botany Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority | ||||
| Abstract | ||||
| THIS STUDY aimed at investigating the effect of gamma irradiation …… on the phenotypic characters of Aspergillus niger and its impact on the activities of some enzymes such as lipase, protease, cellulase, pectinase and amylase. Two phenotypically different mutants were obtained after gamma irradiation at doses of 1 and 3 kGy. SEM microscopy showed clear morphological changes in conidiophores, conidial heads and spores among all strains. The activities of lipase, protease and cellulase in both mutants become less than that of the parent strain, while, pectinase showed no significant difference among the tested strains. Amylase activity was enhanced by gammairradiation in the mutants. The obtained mutants were molecularly characterized using RAPD-PCR. The results demonstrated the occurrence of polymorphic pattern between parent and mutant strains due to change in the genetic makeup. | ||||
| Keywords | ||||
| gamma radiation; Mutagenesis; Aspergillus niger; Fungal Hydrolases | ||||
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