INVESTIGATING THE EFFECT OF MINERAL NUTRITION AND LIGHT QUALITY ON SOME GROWTH-RELATED METABOLITES AND PIGMENTS OF TWO FILAMENTOUS CYANOBACTERIA (CYANOPROKARYOT) | ||||
| Egyptian Journal of Phycology | ||||
| Article 3, Volume 10, Issue 1, 2009, Page 39-57 PDF (444.36 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/egyjs.2009.114840 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Nermin Adel El Semary1; Safia Ghazy1; Abou El Kheir Badawy2; Mohamed El Fouly2; Mohamed Hassoub2 | ||||
| 1Department of Botany and Microbiology, Faculty of Science, Helwan University, Helwan, Egypt | ||||
| 2Fertilization Technology Department, National Research Centre, Dokki, Cairo, Egypt | ||||
| Abstract | ||||
| The effect of mineral nutrition on growth-related metabolites and pigmentation of the filamentous Anabaena flos-aquae and Calothrix sp.PCC7601 was investigated. Key nutrients (iron, phosphorus and nitrogen) were supplied at different concentrations compared to original BG-11 growth medium. The results showed general increase of peptide nitrogen and total sugars, chlorophyll a content and phycobiliprotein pigments content with the use of double concentration of different key nutrients. The opposite was observed for most of these parameters under half and quarter the concentration of nutrients tested in both isolates. Meanwhile, carotenoids increased under half the concentration of iron and phosphorus compared to the original concentrations of BG-11 medium but decreased at quarter concentrations of the original BG-11 medium. The effect of nitrogen limitation on growth and pigments content on A. flos-aquae was overly less severe due to the ability of A. flos-aquae to compensate for limited supply of available nitrogen through nitrogen fixation. At all reduced concentrations of key nutrients, phycoerythrin pigment predominated other phycobiliprotein pigments under white light in Calothrix sp. To further investigate the effect of mineral nutrition and light quality on phycobiliprotein pigment composition, both isolates were grown under green and red monochromatic light. The highest content of phycroerythrin pigment was recorded under green light in both isolatesunder different nutrients treatments. Phycocyanin, on the other hand, pre-dominated other phycobiliprotein pigments in most of nutrients treatments placed under red light in Calothrix sp. This strain was more responsive in terms of alternating its phycobiliprotein composition than A. flos aquae in response to different nutrients concentration and change in light quality. Thus, this isolate seems to be a promising source for those pigments production. The study shows that growth manipulation using different nutrients concentrations and light qualities can result in differential expression of the phycobiliprotein pigments in some cyanobacterial strains. This can be used for mass production of these valuable pigments for biotechnological applications. | ||||
| Keywords | ||||
| Allophycocyanin; Carbohydrate; Carotenoids; Chlorophyll; Chromatic Adaptation; Growth; Phycocyanin; Phycoerythrin; Protein | ||||
| References | ||||
|
Allen, M. M. and Smith, A. J. (1969). Nitrogen chlorosis in blue-green algae. Arch. Microbiol., 69:114-120.
Allen, M. M. and Hutchinson, F. (1980). Nitrogen limitation and recovery in the cyanobacterium Aphanocapsa 6308. Arch. Microbiol., 128:1-7.
Armstrong, J. E. and Van Baalen, C. (1979). Iron transport in microalgae: the isolation and biological activity of a hydroxamate siderophore from the blue-green alga Agmenellum quadruplicatum. Journal of General Microbiology, 111: 253- 262.
Barker-Astrom, K.; Schelin, J.; Gustafsson, P.; Clarke, A. K. and Campbell, D. A. (2005). Chlorosis during nitrogen starvation is altered by carbon dioxide and temperature status and is mediated by the ClpP1 protease in Synechococcus elongatus. Archives of Microbiology, 183: 66–69.
Bei, H. ; Guang-Ce, W. ; Chen-Kui, Z. and Zhen-gang, L. (2002). The experimental research of R- phycoerythrin subunits on cancer treatment - A new photosensitizer in PDT. Cancer Biotherapy and Radiopharmcy, 17:35–42.
Bennett, A. and Bogard, A. (1973). Complementary chromatic adaptation in a filamentous blue-green algae. The Journal of Cell Biology, 58: 419 – 435.
Borowitzka, M. A.; Huisman, J. M. and Osborn, A. (1991). Culture of the astaxanthin-producing green alga Haematococcus pluvialis. I. Effects of nutrients on growth and cell. Journal of Applied Phycology, 3:295-304.
Boussiba, S. and Richmond, A. E. (1980). C-phycocyanin as a storage protein in the blue-green alga Spirulina platensis. Archives of Microbiology, 125:143-147.
Collier, J. L. and Grossman, A. R. (1992). Chlorosis induced by nutrient deprivation in Synechococcus sp. strain PCC 7942: not all bleaching is the same. Journal of Bacteriology, 174: 4718–4726.
Darwish, M. A. (2005). Studies on pigments of some greem algae. Ph.D thesis, Department of Food Science and Technology, Faculty of Agriculture, Al-Azhar University, Egypt.
Datz, G. and Dohler, G. (1981). Light dependent changes in the lipid and fatty acid composition of phycocyanin free photosynthetic lamellae of Synechococcus. Zeitschrift für Naturforschung, 36c: 856-862.
De Loura, I. De Canto.; Dubacq, J. P. and Thomas, J. C. (1987). The effects of nitrogen deficiency on pigments and lipids of cyanobacteria. Journal of Plant Physiology, 83: 838-843.
Dufosse, L.; Galaup, P.; Yaron, A. ; Arad,S.; Blanc, B.; Chidambaramurthy, K.; Ravishanker, G. (2005) Microorganisms and microalgae as source of pigments for use: a scientific oddity or an industrial reality? Trends in Food Science and Technology, 16:389–406.
Emery, T. (1971). Hydroxamic acids of natural origin. Advances in Enzymology, 35:135-155.
Eng-Wilmont, D.L. and Martin, D. F. (1977). Growth response of the marine blue-green alga, Gomphosphaeria aponina, to inorganic nutrients and significance to management. Microbios, 19: 167-179.
Fay, P. (1983). The blue-greens. Studies in Biology/ Institute of Biology; no., 160, Camelot Press, Southhampton, UK.Glazer, A. N. (1994). Phycobiliproteins - a family of valuable, widely used flurophores. Journal of Applied Phycology, 6:105–112.
Grossman, A. R.; Bhaya, D. and He, Q. (2001). Tracking the light environment by cyanobacteria and the dynamic nature of light harvesting. Journal of Biological Chemistry, 276: 11449-11452.
Hardie, L. P.; Balkwill, D. L. and Stevens, S. E. (1983). Effects of iron starvation on the physiology of the cyanobacterium Agmenellum quadruplicatum. Journal of Applied and Environmental Microbiology, 45: 999-1006.
Haselkorn, R.; Golden, J. W.; Lammers, P. J. and Mulligan, M. E. (1989). Developmental rearrangement of cyanobacterial nitrogen–fixation genes. Trends in Genetics, 2:255-259.
Hu, Q.; Sommerfeld, M.; Jarvis, E.; Ghirardi, M.; Posewitz, M.; Seibert, M.; and Darzins, A. (2008). Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances.The Plant Journal, 54: 621–639.
Kaebernick, M.; Nielan, B. A.; Börner, T. and Dittmann, E. (2000). Light and transcriptional response of the microcystin biosynthesis gene cluster. Applied and Environmental Microbiology, 66: 3387-3392.
Kimura, M. (1995). Dyeing with phycocyanin. JP Patent, 166480, 27 June.
Kobayashi, M.; Kakizono, T. and Nagai, S. (1993). Enhanced carotenoid biosynthesis by oxidative stress in acetate-induced cyst ells of a green unicellular alga Haematococcus pulvialis. Applied and Environmental Microbiology, 59: 867-873.
Lowry, O. H.; Rosebrough ,N. J.; Farr, A. L. and Randall, B. J. (1951). Protein measurements with the folin phenol reagent. Journal of Biological Chemistry, 193:265-275.
McKinney, G. (1941). Absorption of light by chlorophyll solutions. Journal of Biological Chemistry, 140:315-322.
Popovic, R.; Martinovic, B.; Vuckovic, M. and Rastovic, A. (1977). The effect of manganese and iron on photosynthetic activity. Biol. Vest., 25: 190.
Rippka, R.; Deruelles, J.; Waterbury, J.B.; Herdman, M. and Stanier, R.Y. (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology, 111: 1-61.
Schwarz, R. and Forchhammer, K. (2005). Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses. Microbiology, 151: 2503-2514.
Sekar, S. and Chandramohan, M. (2008). Phycobiliproteins as a commodity: trends in applied Research. Journal of Applied Phycology, 202: 113-136.
Singh, A. K. and Sherman, L. A. (2000). Identification of ironresponsive, differential gene expression in the cyanobacterium Synechocystis sp. strain PCC 6803 with a customized amplification library. Journal of Bacteriology, 182: 3536–3543.
Stanier, R. Y. and Cohen-Bazire, G. (1977). Phototrophic prokaryotes: the cyanobacteria. Annual Review of Microbiology, 31:225-274.
Umbriet, W. W.; Burris, R. H.; Stauffer, J. F.; Cohen, P. P.; Johnse, W.; Johanse, W. J.; Lee Page, G. A.; Potter, V. R. and Schneider, W.C. (1959). Monometric technique, a manual describing method, applicable to the study of describing metabolism, P. 239. Burgess publishing company.
Verstreate, D. R.; Storch, T. A. and Dunham, V. L. (1980).A comparison of the influence of iron on the growth and nitrate metabolism of Anabaena and Scenedesmus. Physiologia Plantarum, 50: 47-51.
Whitton, B. A. and Potts, M. (2000). The ecology of cyanobacteria. Their diversity in time and space. Freshwater blooms. pp, 149-194. Kluwer Academic Publishers. Dordrecht, The Netherlands. | ||||
|
Statistics Article View: 96 PDF Download: 144 |
||||
