Lipid production and molecular studies of Anabaena torulosa treated with different types of stress | ||||
| Egyptian Journal of Phycology | ||||
| Article 4, Volume 20, Issue 1, 2019, Page 84-101 PDF (906.92 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/egyjs.2019.116023 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Olfat M. A. Salem* 1; Ibtisam A. Hammad1; Fatma A. Badea1; Nagui A. Abdel Khalek2; Khaled A. Selim2 | ||||
| 1Botany and microbiology department, Faculty of Science, Helwan University, Cairo, Egypt. | ||||
| 2Central Metallurgical Research and Development Institute, Cairo, Egypt. | ||||
| Abstract | ||||
| Lipid production in terms of oil percentage and fatty acid composition of Anabaena torulosa which was identified morphologically and genetically was studied to determine its ability for biodiesel production, in response to salinity, nitrogen and phosphorus starvation stress; salinity ranging from 50 to 250mM NaCl, nitrogen and phosphorus regime (50%, NO3-N , 50% PO4-P of BG11 media). The results indicated that there was change in physiological behavior under these different stresses and illustration of these differences genetically. The results of oil analysis revealed that the lipid production increased when nitrogen and phosphorus decrease (68.83% and 160.97% respectively) and 200mM of NaCl increase oil content by 120%. GC/MS analysis of fatty acid composition revealed that methyl linoleate is the main constituent of fatty acids ester 57.9% which classified as a biodiesel due to its long methyl ester chain and is also used as a fuel in standard diesel engines. All these results explained genetically by using RAPD technique which revealed new bands appeared and other bands disappeared. DNA sequence was changed leading to DNA polymorphism 41.25%. Also, the DNA pattern indicated that stress of nitrogen and phosphorus were more related to control, while the other stresses (salinity and combined stresses) caused more changes comparing to control. So that stress changes in physiology was confirmed genetically. | ||||
| Keywords | ||||
| Anabaena torulosa; lipid; fatty acids; salinity; RAPD | ||||
| References | ||||
|
Allen, M.M. (1968).Simple conditions for growth of unicellular blue-green algae. Journal of Phycology, 4:1-4.
Battah, M.G.; El-Ayoty, Y.M.;Esmael, A.E. and Abd El-Ghany, S.E. (2014) Effect of different concentrations of sodium nitrate, sodium chloride and ferrous sulphate on the growth and lipid content of Chlorella vulgaris. Journal of Agricultural Technology, 10 (2): 339-353.
Chakdar, H., and Pabbi, S. (2017) A comparative study reveals the higher resolution of RAPD over ARDRA for analyzing diversity of Nostoc strains. 3 Biotech, 7(2): 125
Chio, H.J.;Joo, J.H.;Joo, H.K.; Wang, P.; Jang, S.K. and Han, M.S. (2018) Morphological characterization and molecular phylogenetic analysis of Dolichospermum hangangense (Nostocales, Cyanobacteria) sp. nov.from Han River, Korea, Algae.,33(2): 143-156.
Cordeiro, R.S.;Vaz, I.C.D.;Magalhães, S.M.S. and Barbosa, F. A.R. (2017) Effects of nutritional conditions on lipid production by cyanobacteria. Anais Da Academia Brasileira De Ciências, 89(3): 2021-2031.
Elsayed, A.B. and Salem, Olfat M.A. (2016) Effect of different light wave lengths on the growth and oil content of Chlorella vulgaris, Egyptian Journal of Phycology, 17: 17-32
Halim, R.; Gladman, B.; Danquah, M.K.and Webley, P.A. (2011) Oil extraction from microalgae for biodiesel production, Bioresource Technology, 102(1): 178-85.
Hu, Q.;Sommerfeld, M.; Jarvis, E.;Ghirardi, M.; Posewitz, M. and Darzins, M.(2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant Journal, 54(4): 621-639.
Keshari, N.; Kumar S.D. and Adhikary S.P. (2015). Identification of cyanobacterial species with overlapping morphological features by 16S rRNA gene sequencing. European journal of phycology, 50(4): 395-399.
Lyra, C.;Hantula J.;Vainio E.;Rapala J.; Rouhiainen L. andSivonen K. (1997). Characterization of cyanobacteria by SDS-PAGE of whole cell proteins and PCR/RFLP of the 16S rRNA gene. Archives of Microbiology., 168 (3): 176-184.
Nirupam, B.;Gour G.S.;SudeshnaS.R.;Nilofer K.;Ramkrishna S.;SanjitK.A.; Prasad R.B.N. and Ruma P. Mapping (2012). Algae of Sundarban Origin as Lipid Feedstock for Potential Biodiesel Application. Journal of algal biomass utilization.3 (2): 42- 49.
Okore, K.;Nwaehiri, L.; Mbanefo,O.;Ogbulie, T. and Ogbuka, I.(2017). The use of random amplified polymorphic DNA (RAPD) to study the genetic variation of biosurfactant producing bacteria. Academic Journal of Science.7(3): 267–286.
Perumal, G.M.;Ganesan, V. and Anand, N. (2009). Identification and phylogenetic analysis of filamentous Cyanobacteria using random amplified polymorphic DNA (RAPD) fingerprinting. African Journal of Biotechnology, 8(6): 974-978.
Prasanna, R.; Kumar, R.;Sood, A.;Prasanna, B.M. and Singh, P.K. (2006).Morphological, physiochemical and molecular characterization of Anabaena strains. Microbiological Research, 161: 187-202.
Rodolfi, L.;Chini, Z.G.;Bassi, N.;Padovani, G.;Biondi, N.;Bonini, G. and Tredici, M.R. (2009). Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor., Biotechnology and Bioengineering., 102(1): 100-120.
Rogers, S.O. and Bendish, A.J. (1998).Extraction of DNA from plant tissues. In: Gelvin SB, Schilperoort RA (eds). Plant molecular biology manual. Kluwer Academic Publishers, Dordrecht/Boston/London, 1-10.
Rozes, N.;Garbay, S.;Denayrolles, M. and Lonvaud-Funel, A. (1993).A rapid method for the determination of bacterial fatty acid composition. Letters in Applied Microbiology, 17(3): 126-131.
Salehzadeh, A. and Naeemi, A.S. (2017).Biodiesel production from Anabaena variabilis cyanobacterium. Indian Journal of Geo Marine Sciences, 46 (2): 385-390.
Salem, M.A.O.; El-Ardy, O. and Abd El-Rahman, M. A. (2013). Effect of nitrogen and phosphorus concentrations in growth medium and Salt stress on growth, lipid content, and biodiesel producing ability of microalgae, Egypt.J.Bot.3rd international conf.17-18 April, Helwan Univ.219-233.
Sallal, A. K.; Nimer, N. A. and Radwan, S. S. (1990).Lipid and fatty acid composition of freshwater cyanobacteria, Journal of General Microbiology, 136: 2043-2048.
Shalini, A.;Tiwari, S.; Gupta, R.K.;Pabbi S. and Dhar, D.W. (2007(. Protocol optimization for RAPD in cyanobacteria. Indian Journal of Biotechnology, 6: 549-552.
Shuaib, M.; Zeb, A.; Ali, Z.; Ali, W.; Ahmad, T. and Khan, I.(2007). Characterization of Wheat Varieties by Seed Storage Protein Electrophoresis. African journal of biotechnology, 6: 497-500.
Takagi, M.; Karseno, S. and Yoshida, T. (2006).Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells. Journal of Bioscience and Bioengineering, 101(3): 223-226.
Tang, D.; Han, W.; Li, P.; Miao, X.andZhong, J. (2011). CO2biofixation and fatty acid composition of Scenedesmusobliquus and Chlorella pyrenoidosa in response to different CO2 levels, Bioresource Technology.102 (3): 3071-3076.
Valerio, E.; Chambel, L.;Paulino, S.;Faria, N.; Paulo, P. and Roge´rio T. (2009).Molecular identification, typing and traceability of cyanobacteria from freshwater reservoirs.Microbiology,155: 642-656.
Wacklin, P.; Hoffmann, L. and Komárek J. (2009). Nomenclatural validation of the genetically revised cyanobacterial genus Dolichospermum (Ralfs ex BornetetFlahault) comb. nova, Fottea.9 (1): 59-64.
Whitton,B.A. and Potts, M. (2012). Introduction to the Cyanobacteria. In: The ecology of cyanobacteria II. Their diversity in space and time (Whitton, B.A. editor).Springer, Dordrecht.1-13. | ||||
|
Statistics Article View: 232 PDF Download: 218 |
||||
