The Viability and Cytotoxic Effect of High Mobility Group Box Protein 1 on MC3T3-E1 Pre-osteoblast Cells: an In Vitro Study | ||||
| Zagazig Veterinary Journal | ||||
| Article 3, Volume 48, Issue 3, September 2020, Page 254-262 PDF (413.59 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zvjz.2020.27091.1106 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ahmed Monir  1; Abdel Basit Abd El-Aal2; Mohamed Omar2; Mahasen El-Shair2; Taro Mukaibo3; Ryuji Hosokawa4
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| 1Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Zagazig University, Sharkia, 44511, Egyptt | ||||
| 2Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Zagazig University, Sharkia, 44511, Egypt | ||||
| 3Department of Oral Reconstruction and Rehabilitation, Kyushu Dental University, Kitakyushu, Fukuoka, Japan | ||||
| 4Department of Oral Reconstruction and Rehabilitation, Kyushu Dental University, Kitakyushu, Fukuoka, Japan. | ||||
| Abstract | ||||
| Osteoblast proliferation and migration are significantly involved in bone healing, regeneration and embryonic skeletal development. Recently, reports suggest anabolic properties of High Mobility Group Box Protein 1 (HMGB1) within the subsequent phase of tissue regeneration as well as HMGB1 has been shown to be highly expressed during bone fracture and regulates osteochondral ossification. In this study, the effect of various concentrations of Recombinant Human HMGB-1 (rhHMGB-1) (50, 100,150 and 200 µg/L) on the viability and proliferation of Pre-osteoblast cells MC3T3-E1 were examined over 24, 48 and 72 hours intervals in comparison with control, untreated cells. A cell count kit (CCk-8) was used to assess osteoblast viability and proliferation, results were quantified using a microplate reader and the viability %( survival rate) was calculated. All experiments were done in triplicate and outcomes were statistically analyzed. A direct positive impact of HMGB-1 on the viability and proliferation of osteoblast cells was in a dose dependent manner. Lower concentration of HMGB-1 (50 and 100 µg/L) showed a non-significance difference in the viability and proliferation of osteoblast cells over time, however, a higher concentration of HMGB-1 (150 and 200 µg/L) showed a significant increase in the proliferation and survival rate by 1.7 fold compared to control group in the 2nd and 3rd day of treatment. No significant difference was found between the 150 and 200 µg/L concentrations which suggested the appropriate dose will be 150 µg/L. Moreover, further in vivo and in vitro investigations will be required. | ||||
| Keywords | ||||
| High mobility group box protein-1(HMGB-1); osteoblast; Viability; Proliferation; MC3T3-E1 pre-osteoblast cells | ||||
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