SELECTION OF SALT TOLERANT CLONES OF SOME MEDICINAL PLANTS THROUGH TISSUE CULTURE TECHNIQUE: I. Hyoscyamus muticus L. PLANT.
Hanna, N., El-Gamassy, K., Kandeel, A., Helmy, L. (2009). SELECTION OF SALT TOLERANT CLONES OF SOME MEDICINAL PLANTS THROUGH TISSUE CULTURE TECHNIQUE: I. Hyoscyamus muticus L. PLANT.. EKB Journal Management System, 34(3), 1869-1879. doi: 10.21608/jpp.2009.116949
N. E. Hanna; K. M. El-Gamassy; A. M. Kandeel; Laila M. Helmy. "SELECTION OF SALT TOLERANT CLONES OF SOME MEDICINAL PLANTS THROUGH TISSUE CULTURE TECHNIQUE: I. Hyoscyamus muticus L. PLANT.". EKB Journal Management System, 34, 3, 2009, 1869-1879. doi: 10.21608/jpp.2009.116949
Hanna, N., El-Gamassy, K., Kandeel, A., Helmy, L. (2009). 'SELECTION OF SALT TOLERANT CLONES OF SOME MEDICINAL PLANTS THROUGH TISSUE CULTURE TECHNIQUE: I. Hyoscyamus muticus L. PLANT.', EKB Journal Management System, 34(3), pp. 1869-1879. doi: 10.21608/jpp.2009.116949
Hanna, N., El-Gamassy, K., Kandeel, A., Helmy, L. SELECTION OF SALT TOLERANT CLONES OF SOME MEDICINAL PLANTS THROUGH TISSUE CULTURE TECHNIQUE: I. Hyoscyamus muticus L. PLANT.. EKB Journal Management System, 2009; 34(3): 1869-1879. doi: 10.21608/jpp.2009.116949

SELECTION OF SALT TOLERANT CLONES OF SOME MEDICINAL PLANTS THROUGH TISSUE CULTURE TECHNIQUE: I. Hyoscyamus muticus L. PLANT.

Journal of Plant Production
Article 8, Volume 34, Issue 3, March 2009, Page 1869-1879  XML PDF (456.25 K)
Document Type: Original Article
DOI: 10.21608/jpp.2009.116949
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Authors
N. E. Hanna; K. M. El-Gamassy; A. M. Kandeel; Laila M. Helmy
Hort. Dept., Fac. Agric., Ain shams Univ., Shoubra El-Keima, Cairo, Egypt.
Abstract
Response of Egyptian henbane plant callus (Hyoscyamus muticus L.) to various levels of salt was investigated. Two tolerant clones to salt were selected from callus cultured on selection medium contained 6000 and 8000 ppm of salts, respectively. Tolerant clones were capable of proliferating and growing on media contained the same salt concentrations and supplemented with 1.5 ppm of BA. Vegetative characters were significantly enhanced in general at the lower salt concentration (6000 ppm) especially with the media containing 0.12 g/ L KH2PO4 +4 ppm kinetin + 0.5 ppm IBA. Using of MS media at its half strength with 1ppm NAA was the best for henbane root growth at 6000ppm of salt. Survival percentage  reached  up to 28.00 % for clone A(selected  at 6000ppm ) and 32.02 % for clone  B(selected  at 8000ppm) with a total alkaloid percentage of  0.90  and 0.98 %, respectively .
Keywords
Salt tolerant clones; Tissue culture technique; Hyoscyamus muticus
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