BOTANICAL STUDIES ON THE MICROPROPAGATION OF TWO DRIED CV(S) FROM DATE PALM (phoenix dactylifera, L.) USING TISSUE CULTURE TECHNIQUE. 2-CARBOHYDRATES FRACTIONS AND ACCLIMATIZATION.
Helaly, M., Hegazy, A., El-Hosieny, H. (2009). BOTANICAL STUDIES ON THE MICROPROPAGATION OF TWO DRIED CV(S) FROM DATE PALM (phoenix dactylifera, L.) USING TISSUE CULTURE TECHNIQUE. 2-CARBOHYDRATES FRACTIONS AND ACCLIMATIZATION.. EKB Journal Management System, 34(3), 2041-2056. doi: 10.21608/jpp.2009.116979
M. N. Helaly; A. E. A. Hegazy; Hanan A. M. El-Hosieny. "BOTANICAL STUDIES ON THE MICROPROPAGATION OF TWO DRIED CV(S) FROM DATE PALM (phoenix dactylifera, L.) USING TISSUE CULTURE TECHNIQUE. 2-CARBOHYDRATES FRACTIONS AND ACCLIMATIZATION.". EKB Journal Management System, 34, 3, 2009, 2041-2056. doi: 10.21608/jpp.2009.116979
Helaly, M., Hegazy, A., El-Hosieny, H. (2009). 'BOTANICAL STUDIES ON THE MICROPROPAGATION OF TWO DRIED CV(S) FROM DATE PALM (phoenix dactylifera, L.) USING TISSUE CULTURE TECHNIQUE. 2-CARBOHYDRATES FRACTIONS AND ACCLIMATIZATION.', EKB Journal Management System, 34(3), pp. 2041-2056. doi: 10.21608/jpp.2009.116979
Helaly, M., Hegazy, A., El-Hosieny, H. BOTANICAL STUDIES ON THE MICROPROPAGATION OF TWO DRIED CV(S) FROM DATE PALM (phoenix dactylifera, L.) USING TISSUE CULTURE TECHNIQUE. 2-CARBOHYDRATES FRACTIONS AND ACCLIMATIZATION.. EKB Journal Management System, 2009; 34(3): 2041-2056. doi: 10.21608/jpp.2009.116979

BOTANICAL STUDIES ON THE MICROPROPAGATION OF TWO DRIED CV(S) FROM DATE PALM (phoenix dactylifera, L.) USING TISSUE CULTURE TECHNIQUE. 2-CARBOHYDRATES FRACTIONS AND ACCLIMATIZATION.

Journal of Plant Production
Article 20, Volume 34, Issue 3, March 2009, Page 2041-2056  XML PDF (776.86 K)
Document Type: Original Article
DOI: 10.21608/jpp.2009.116979
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Authors
M. N. Helaly1; A. E. A. Hegazy2; Hanan A. M. El-Hosieny1
1Agric Bot . Dep. Faculty of Agric Mansoura Univ., Egypt
2Genetic engineering Inst and Bio- technology Menofia Univ.
Abstract
Bartamuda cv has higher level of all carbohydrates fractions studied and least values of total proteins, amino acids and proline concentrations compared with Sakkoty cv overall the developmental stages. The possibility that Sakkoty cv can form specific protein types for adaptation was suggested.
The concentrations of reducing sugars, non reducing sugars and total sugars were increased whereas total protein and amino acids were decreased with subculturing. The lowest significant values of carbohydrate fractions and the highest values of total protein , amino acids and proline were noticed at the embryogenic stage. Polysaccharides and insoluble sugars were decreased gradually in Bartamuda cv. whereas in Sakkoty cv, up to the embryonic callus and thereafter increased .
The interaction treatments showed that, the highest significant value of carbohydrates fractions were recorded with Bartamuda cv at initial explant and callus stages in addition to Sakkoty cv at only the callus stage. The lowest significant values were found with Sakkoty cv at the embryogenic stage. At plantlet stage, a slight increases in all carbohydrates formations concentrations were noticed, but still less than the corresponding calculated values recorded at stages of the initial shoot tip explants and callus initiations.Total protein and amino acids were decreased at callus stage gradually up to the  embryogenic callus and thereafter increased at plantlet stage to reached vales more than the corresponding initial explant. These results are true in the two cv (s) studied.
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