EFFECT OF GENOTYPE, EXPLANT AND KINETIN CONCENTRATIONS ON SHOOT REGENERATION AND EVALUATION OF SALINITY TOLERANCE IN TOMATO | ||||
| Journal of Plant Production | ||||
| Article 6, Volume 34, Issue 5, May 2009, Page 4761-4776 PDF (899.07 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2009.117286 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Sahar S. Taha* | ||||
| Vegetable Crops Department. Faculty of Agriculture. Cairo University | ||||
| Abstract | ||||
| In this study, four tomato hybrids (Sarya, Nematoda, Mereto and Abeza )and four wild species (L. pimpinellifolium PI344102, L. peruvianum CMV-INRA, L. escu. PI174263 and L. escu. var. ceriaciforme PI321749) were used. Hypocotyl and cotyledon explants were isolated from seedling and cultured on modified MS medium (Murashige and Skoog ,1962 ),which contained MS salts and B5 vitamins (Gamborg et al., 1968), 1% (w/v) agar supplemented with kinetin at levels 0.5 , 1.0 and 2.0 mg/l. The highest percentage of callus was produced in cv. Abeza and L.pimpin.PI344102. The highest number of explants that produced shoots was observed in L. escu. PI174263on MS media with 1.0 and 2.0 mg/l KIN. Maximum total number of shoots and number of shoots per explant was produced by culturing cotyledon explants of L. escu. PI 174263 on MS media with 2.0 mg/l KIN. Tomato seeds ( L. pimpin. PI344102, L. peruv. CMV-INRA, L. escu. PI174263 and L. escu. var. ceriaciforme PI321749) were cultured on MS medium with 2.0 mg/l KIN and supplemented with different concentrations of sea salt (0.0, 2000, 4000, 6000 and 8000 ppm ). The germination percentage and plant fresh weight was the highest in L.escu. PI174263. Tallest plants were produced in L.escu. PI174263and L.escu. var. ceriaciforme PI3217. Increasing salinity reduced germination percentage, plant height, leaves number and plant fresh weight in all genotypes, except in L. Pimpin. were increasing the salinity upto 4000 ppm increased plant height. | ||||
| Keywords | ||||
| Tomato; Organogenesis; Regeneration; Explant; Media and Salinity | ||||
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