Evaluation of Regulatory T cells in Obese Asthmatic Children | ||||
| The Egyptian Journal of Pediatric Allergy and Immunology | ||||
| Article 6, Volume 18, Issue 2, October 2020, Page 79-85 PDF (264.6 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejpa.2020.25415.1004 | ||||
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| Authors | ||||
Magdy Zedan1; Nanees Abdelbadie Salem2; Mohamed Magdy Zedan2; Hoda Nasser Abbass  3; Layla Saleh4
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| 1Professor of Pediatrics, Pediatric Department, Mansoura Faculty of Medicine, Mansoura University, Mansoura, Egypt | ||||
| 2Assistant Professor of Pediatrics Faculty of Medicine - Mansoura University | ||||
| 3Faculty of medicine Mansoura university | ||||
| 4Lecturer of Clinical Pathology Faculty of Medicine - Mansoura University | ||||
| Abstract | ||||
| Background: Asthma is an airway inflammatory disorder with functional and structural changes. Regulatory T (T reg) cells are important in controlling immune responses. The study was aimed to investigate the frequency of Treg cells in obese asthmatic children, in comparison to non-obese asthmatics and healthy matched controls . Methods: In addition to anthropometric and body mass index (BMI) assessment, peripheral blood samples from healthy control subjects (n = 30) and asthmatic obese (n=30) and asthmatic non-obese children (n=30) were examined for serum IgE, eosinophils counts, and flowcytometric measurement of CD4+CD25+CD127 low/neg- T cells. Pulmonary function testing was performed for asthmatic children . Results: Obese asthmatics showed significantly higher levels of serum IgE and CD4+CD25+CD127 low/neg- T cells as compared to healthy controls (p < 0.001, 0.001, respectively) while comparable numbers of T reg cells were found among obese and non-obese asthmatic children. Asthmatics receiving inhaled corticosteroids (ICS) showed higher percentages of CD4+CD25+CD127 low/neg- T cells than the non-receivers (median 11.8% vs 8.8%, p <0.001). No significant correlations were found between Treg and age, eosinophil percentage, total serum IgE, pulmonary functions, or BMI and its Z score . Conclusion: Our study demonstrates an increased frequency of Treg cells in asthmatic children compared to controls with possible association with the use of ICS but not with obesity. Small sample size and lack of obese non-asthmatic group are the main points of limitation in our study. | ||||
| Keywords | ||||
| asthma; natural regulatory T cells; FOXP3; obesity | ||||
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