Efficacy of PCR-bassed techniqes and remote sensing technology for detecting potato brown rot infection caused by R. solanacearum
BALABEL, N., Mamdouh, A., Younes, S. (2020). Efficacy of PCR-bassed techniqes and remote sensing technology for detecting potato brown rot infection caused by R. solanacearum. EKB Journal Management System, 98(2), 240-269. doi: 10.21608/ejar.2020.118061
NAGLAA BALABEL; Ali M. Mamdouh; Shams Nour El-Dein Younes. "Efficacy of PCR-bassed techniqes and remote sensing technology for detecting potato brown rot infection caused by R. solanacearum". EKB Journal Management System, 98, 2, 2020, 240-269. doi: 10.21608/ejar.2020.118061
BALABEL, N., Mamdouh, A., Younes, S. (2020). 'Efficacy of PCR-bassed techniqes and remote sensing technology for detecting potato brown rot infection caused by R. solanacearum', EKB Journal Management System, 98(2), pp. 240-269. doi: 10.21608/ejar.2020.118061
BALABEL, N., Mamdouh, A., Younes, S. Efficacy of PCR-bassed techniqes and remote sensing technology for detecting potato brown rot infection caused by R. solanacearum. EKB Journal Management System, 2020; 98(2): 240-269. doi: 10.21608/ejar.2020.118061

Efficacy of PCR-bassed techniqes and remote sensing technology for detecting potato brown rot infection caused by R. solanacearum

Egyptian Journal of Agricultural Research
Article 4, Volume 98, Issue 2, July 2020, Page 240-269  XML PDF (1.56 MB)
Document Type: Original Article
DOI: 10.21608/ejar.2020.118061
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Authors
NAGLAA BALABEL email 1; Ali M. Mamdouh2; Shams Nour El-Dein Younes3
1Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza, Egypt. Potato Brown Rot Project (PBRP), Ministry of Agriculture and Land Reclamation, Dokki, Egypt.
2Geology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt.
3Department of Geography, Faculty of Arts, Cairo University, Giza, Egypt.
Abstract
Bacterial wilt of potatoes (Solanum tuberosum) caused by Ralstonia solanacearum phylotype II sequevar I (race 3 biovar 2), is one of the most serious diseases of potatoes. This study aimed to detect such deteriorations through the remotely sensed data. In this regard, naturally infested potato fields at Abo Alkhawi, Al Tayareya and Om Saber villages at Behera governorate, Egypt, were used for selective isolation of R. solanacearum. On Semi Selective South Africa (SMSA) medium, 30 isolates were randomly screened and tested by immunofluorescence antibody staining (IFAS) and confirmed biologically by tomato plantlets (cv. Pinto) reaction. All isolates showed positive results with Real-time PCR. Based on the phylotype specific multiplex (Pmx) – PCR data, all isolates in concern were R. solanacearum and affiliated to phylotype II as 372- bp amplicon.
Spectral indices were applied on the Landsat 8 and RapidEye satellite images acquired in January and April 2019 including Normalized Difference Moisture Index (NDMI), Normalized Difference Vegetation Index (NDVI), Normalized Difference Salinity Index (NDSI) and Modified Chlorophyll Absorption Ratio Index (MCARI). Results from the remote sensing analyses showed medium to relatively high salinity and moisture content of soils concerened. Moreover, plants chlorophyll levels are in medium levels which is not typical to condition associated with usual circumstances. Further investigation, in a greater academic depth, are needed for pathological field surveys.
 
Keywords
SMSA medium; immunofluorescent antibody staining (IFAS); Phylotype analysis; Real-time PCR; wilt and brown rot diseases; indices; Landsat 8; RapidEye; soil salinity; moisture content and chlorophyll content
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