Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen
Leil, A., A. El-Shamy, A., D.A., E. (2020). Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen. EKB Journal Management System, 5(4), 61-67. doi: 10.21608/javs.2020.118167
Amal Z Leil; Ayat A. El-Shamy; El-Badry D.A.. "Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen". EKB Journal Management System, 5, 4, 2020, 61-67. doi: 10.21608/javs.2020.118167
Leil, A., A. El-Shamy, A., D.A., E. (2020). 'Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen', EKB Journal Management System, 5(4), pp. 61-67. doi: 10.21608/javs.2020.118167
Leil, A., A. El-Shamy, A., D.A., E. Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen. EKB Journal Management System, 2020; 5(4): 61-67. doi: 10.21608/javs.2020.118167

Clinical Application Of Intrauterine Laparoscopic Insemination In Zaraiby Goats Using Different Concentrations Of Zaraiby And Boer Bucks’ Frozen Semen

Journal of Applied Veterinary Sciences
Article 8, Volume 5, Issue 4, October 2020, Page 61-67  XML PDF (692.86 K)
Document Type: Original Article
DOI: 10.21608/javs.2020.118167
View on SCiNiTO View on SCiNiTO
Authors
Amal Z Leil1; Ayat A. El-Shamy2; El-Badry D.A. email 3
1Department of Field Investigation, Animal Reproduction Research Institute, Agriculture Research Center (ARC), Giza, Egypt
2Artificial Insemination and Embryo Transfer Dept., Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt.
3Dept. Artificial Insemination and Embryo Transfer, Animal Reproduction Research Institute, Agriculture Research Center. Giza, Egypt
Abstract
This study aimed to determine the proper sperm dose per laparoscopic intrauterine insemination (LAI) in Zaraiby goats.  Thirty-six Zaraiby goats, two Zaraiby and two Boer bucks were used in the study. Does were divided into six groups; the first three groups were inseminated laparoscopically with frozen-thawed Zaraiby semen either at doses of 10 x 106 (GP1), 20 x 106 (GP 2), or 40 x 106 (GP 3).  The other three groups were inseminated with Boer semen's same doses (GP 4, 5 &6).  Results showed that goats laparoscopically inseminated with 20 x 106 Zaraiby spermatozoa or 10 x 106 Boer sperm cells both had the highest (P ≤0.05) pregnancy rate (about 70% & 60%, respectively).  The multiple birth rate in goats inseminated by Boer buck’s spermatozoa were generally higher than those inseminated with Zaraiby buck’s spermatozoa (66.67 to 100.00% vs. 25.00 to 40.00%, respectively).  In conclusion, the LAI is an efficient means of achieving high fertilization rates in goats.  The recommended minimum necessary dose for laparoscopic artificial insemination in Zaraiby is 10 ×106 and 20×106 motile Boer and Zaraiby buck’s spermatozoa, respectively. 
Keywords
Goat; insemination; laparoscopic; semen dose; Zaraiby
References
ANAKKUL, N., SUWIMONTEERABUTR, J., THARA-SANIT, T., KHUNMANEE, S., DILOKSUMPAN, P., DEBRA K. BERG, TECHAKUMPHU, M., 2014. Sperm distribution and fertilization after unilateral and bilateral laparoscopic artificial insemination with frozen-thawed goat semen.  Theriogenology 82:1137–1144.

ANAKKUL, N., SUWIMONTEERABUTR, J., THARA-SANIT1, T. PANYABORIBAN, S., KHUNM-ANEE, S., THANOMSUKSINCHAI, N., TECHAKUMPHU , M., 2013. Production of black goat using laparoscopic artificial insemination and embryo transfer. Thai J Vet Med.43(2): 259-263.

BARBAS, J. P., and MASCARENHAS, R. D.,2009. Cryopreservation of domestic animal sperm cells. Cell Tissue Bank 10: 49–62.

BONATO, GC, MAIA, ALRS, CÔRTES, LR, OLIVEIRA, TA, ARRAIS, A.M., FIGUEIRA, LM, OLIVEIRA, MEF, SOUZA-FABJAN, J.M.G.a and FONSECA, JF.,2019. Effects of d-cloprostenol administrations with 7.5 and 11.5-day intervals between administrations on pregnancy rates after artificial insemination in estrous cyclic dairy goats.  Animal Reproduction Science 209: 106-172.

CSEH, S., FAIGL, V. and AMIRIDIS, G.S., 2012. Semen processing and artificial insemination in health management of small ruminants. Anim Reprod Sci. 130(3-4):187-192.

DE GRAAF, SP, BEILBY, K., O'BRIEN, JK, OSBORN, D., DOWNING, JA, MAXWELL, WM and EVANS, G., 2007. Embryo production from superovulated sheep inseminated with sex-sorted ram spermatozoa. Theriogenology 67: 550–555.

DICKSON, U.L., RANGEL, R., TORRES, G., BECERRIL, C. and GONZALES, F., 2001. Fertilidad Y Prolifi Cidad De Cabras Alpino Frances Y Saanen Inseminadas Con Semen Congelado Importado. In: II Congreso Latinoamericano de Especialistas en Pequneos Rumiantes y Camėlidos Sudamericanos. IX Congreso Nacioal de Producciŏn Ovina. Mĕrida, Yuc., Mexico.

EL-KHAWAGAH, ARM, RAWASH, ZM, EL-BADRY, DA and KANDIEL, MMM.,2020. Influence of butylated hydroxytoluene addition to cryodiluents on freezability and DNA integrity of Boer and Zaraiby buck spermatozoa. Asian Pac J Reprod. 9(2): 96-103.

EPPLESTON, J., MAXWELL, WMC, BATTYE, KM and ROBERTS, EM., 1986. Effect of thawed motility and intrauterine dose of motile sperm on the fertility of ewes. Proc. Aust. Sot. Reprod. Biol. 18: 19 Abstr.

EPPLESTON, J., SALAMON, S., MOORE, N. and EVANS, G. 1994.The depth of cervical insemination and site of intra-uterine insemination and their relationship to the fertility of frozen-thawed ram semen. Anim. Reprod. Sci. 36:21l–25.

EVANS, G. AND MAXWELL, WMC,1986: Salamon's Artificial Insemination of Sheep and Goats.  Butterworth, Sydney.

EVANS, G. AND MAXWELL, WMC, 1987. Salamon’s Artificial Insemination in Sheep and Goats. Sydney: Butterworths.

FAIGL, VERA, NÓRA, V., JÁVOR, A., KULCSÁR, M., SOLTI, L., GEORGIOS AMIRIDIS N.D. and CSEH, S., 2012. Artificial insemination of small ruminants –a review.  Acta Veterinaria Hungarica60 (1); 115–129.

FONSECA, FERREIRA, ALVIM, GP, GONÇALVES SOUZA-FABJAN, JM, OLIVEIRA, MEF, BRAIR, VL BRANDÃO, ZF and JEFERSON, OF., 2017. Reproductive features and use of an anti-inflammatory drug in estrus-induced dairy goats artificially inseminated in a standing position with cervix immobilization. Reprod Biol.  17(3):268-273.

GIBBONS, A.E., FERNANDEZ, MARÍA MACARENA BRUNO-GALARRAGA, MARÍA VICTORIA SPINELLI, MARCELA ISABEL CUETO, 2019. Technical recommendations for artificial insemination in sheep. Anim Reprod. 16(4):803-809.

GOONEWARDENE, L., WHITMORE, W., JAEGER, S., EMOND, S., 1997. Effect of prebreeding maintenance diet on artificial insemination in Alpine and Saanen goats. Theriogenology. 48: 151-159.

KHALIFA, TAA, 2001. Effect of some antioxidants on viability of preserved buffalo and ram semen. Ph.D. Thesis, Fac. Vet. Med. Cairo Univ.

KULAKSIZ, R. and A.R.I, U.C., 2016. Correctness/Incorrectness on Laparoscopic Artificial Insemination in Sheep and Goats.  Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics 2(1):38-47.

KULAKSIZ, RECAI and DAŞKIN, A., 2012. Reproductive performance of primiparous and multiparous Saanen goats after laparoscopic intrauterine insemination: a field study Turk. J. Vet. Anim. Sci. 36(2): 201-204

KUMAR, D. and NAQVI, SMK., 2014. Effect of time and depth of insemination on fertility of Bharat Merino sheep inseminated trans-cervical with frozen-thawed semen. J Anim Sci Technol. 56(1):8.

LEBOEUF, B., RESTALL, B. and SALOMON, S., 2000. Production and storage of goat semen for artificial insemination. Anim Reprod Sci. 62: 113–141.

LÓPEZ-GATIUS, F., 2000.Site of semen deposition in cattle: a review. Theriogenology 53: 1407-1414.

LOWINGER, P.M., ROBERI, I., COLATTO, C., BOERIS, M. and YAFUL, G., 2001. Programa Piloto De Inseminacion Artifi Cial En Cabras En El Departamento De Malargue, Provincia De Mendoza, Argentina. IN: II Congreso Latinoamericano de Especialistas en Pequneos Rumiantes y Camėlidos Sudamericanos. IX Congreso Nacioal de Producciŏn Ovina. Mĕrida, Yuc., Mexico.

LU, CD.,2001. Boer Goat Production: Progress and Perspective. Proceedings of International Conference on Boer Goats. Anshun, China. 1-11.

Martínez-Rojero, R.D., Hernández, J., Reyna, L. and Santos R., 2007. Intrauterine insemination with frozen-thawed semen in Creole goats synchronized in estrous during the nonbreeding season. Res J Anim Sci.1:76–80.

MOORE, RW, MILLER, CM and HALL, D.R., 1988. Cervical versus laparoscopic AI of a goat after PMSG injection at or 48 hours before CIDR removal. Proc. New Zealand Soc. Anim. Prod. 48: 69–70.

MORRIS, LHA and ALLEN, W.R., 2002.An overview of low dose insemination in the mare. Reprod. Domest. Anim. 37: 206–210.

PALACÍN, I., YÁNIZ, J.L., FANTOVA, E., BLASCO, M.E., QUINTÍN-CASORRÁN, F.J., SEVILLA-MUR, E., and SANTOLARIA, P., 2012. Factors affecting fertility after cervical insemination with cooled semen in meat sheep. Anim Reprod Sci. 132(3-4):139-44.

RITAR, AJ and SALAMON, S.,1983.Fertility of fresh and frozen-thawed semen of the Angora goat. Aust. J. Biol. Sci., 36: 49–59.

RITAR, AJ, BALL, PD and O'MAY, PJ., 1990. Artificial insemination of Cashmere goats: Effect of fertility and fecundity of intravaginal treatment, method and time insemination, semen freezing process, number of motile spermatozoa and age of females. Reprod Fertil Dev. 2(4):377-84.

SALAMON, S. AND EVANS, G. AND MAXWELL, WMC.,1985. Fertility of ram semen frozen-stored for 16 years. Ann. Conf. Aust. SOC. Reprod. Bio. l: 62, Abstrac.

SALAMON, S. and MAXWELL, M. C., 1995. Frozen storage of ram semen. II. Causes of low fertility after cervical insemination and methods of improvement. Anim. Reprod. Sci. 38: 1–36.

SALAMON, S. and MAXWELL, WMC., 2000. Storage of ram semen. Animal Reproduction Science 62: 77–111.

SCENNA, FN, HOCKETT, ME, TOWNS, TM, SAXTON, A.M., ROHRBACH, NR, WEHRMAN, ME, et al., 2005. Influence of a prostaglandin synthesis inhibitor administered at embryo transfer on pregnancy rates of recipient cows. Prostaglandins Other Lipid Mediat 78:38–45.

SNEDECOR, GW and COCHRAN, WG., 1989. Statistical Methods. 8th Ed. Iowa State Univ. Press, Ames, IA, USA.

SOHNREY, B. and HOLTZ, W., 2005. Technical Note: Transcervical deep corneal insemination of goats. J Anim Sci. 83: 1543-1548.

TEKIN, K., 2019. Cervical Insemination with Frozen Thawed Semen in Goats at Different Breeding Age. Kocatepe Vet J. 12(3):357-362.

TONI, D., PLAMEN, T., VLADIMIR, P., FLORINA, P., LJUPCE, K. and JURAJ, G., 2012. Laparoscopy-promising tool for improvement of reproductive efficiency of small ruminants. Mac. Vet. Rev.  35 (1): 5–11.

VERBERCKMOES, S., VAN SOOM, A., DE PAUW, I., DEWULF, J., VERVAET, C. and DE KRUIF, A., 2004. Assessment of a new utero-tubal junction insemination device in dairy cattle. Theriogenology 61: 103–115.

WATSON, PF and BEHAN, JR., 2003. Intrauterine insemination of sows with reduced sperm numbers: Results of a commercially based field trial. Theriogenology 57:1683–1693.

WULSTER-RADCLIFFE, M. C., WANG, S. and LEWIS, G. S., 2004. Transcervical artificial insemination in sheep: Effects of a new transcervical insemination instrument and traversing the cervix on pregnancy and lambing rates. Theriogenology 62: 990–1002.

YOTOV, S., ATANASOV, A., KARADAEV, M., DIMOVA, L. and VELISLAVOVA, D., 2016. Pregnancy rate in dry and lactating goats after estrus synchronization with artificial insemination and natural breeding (a field study). Bulgarian Journal of Veterinary Medicine 19(3): 218–223.

Statistics
Article View: 1,734
PDF Download: 1,057