Impact of Kinetin and Benzyladenine on Growth Performance of Croton in Vitro | ||||
Alexandria Science Exchange Journal | ||||
Article 7, Volume 41, July-September - Serial Number 3, September 2020, Page 381-391 PDF (571.47 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/asejaiqjsae.2020.118280 | ||||
View on SCiNiTO | ||||
Authors | ||||
Hoda I. M. El-Gedawey* 1; Ali I. H. Abido* 2; Mohamed K. Gaber2 | ||||
1Agriculture research center, Horticulture Institute, Ministry of Agric., Deprt. of Ornamental and Landscape Gardening, Antoniadis, Alex. | ||||
2Plant Production Depit., the Faculty of Agric-saba Basha, Alex.univ | ||||
Abstract | ||||
This study was carried out in the tissue culture laboratory, Faculty of Agric., Saba Basha, Alex. Univ. to fined reliable protocol for in vitro propagation of Croton (Codiaeum variegatun L.) during the period from 2019 to 2020 was developed. Moreover, nodal explants were used during in vitro culture study for indication of multiple shoots and inoculated on various media with different combinations of NAA as auxin and (BA & KIN) as cytokinin to study compare the effect of the two types of cytokinins on proliferation and development of multiple shoots, and the elongation of the new formed on medium. The best medium for multiplication was a medium supplemented with 2.00mg/l KIN and (0.50 or 1.00) mg/l NAA. Furthermore, the in vitro shoots showed healthy root development when the tested medium was supplemented with combination of 1.00mg/l KIN and NAA each, in turn. The new formed shoot plantlets (rooted plants) were acclimization ex vitro successfully. The survival rate of the ex vitro grown plants was 95%. | ||||
Keywords | ||||
in vitro culture; Codiaeum variegatun L; kinetin; benzyle adenine | ||||
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