Detection of Brucella organisms from Egyptian raw milk using cultural and molecular techniques
Aman, I., AL-Hawary, I., Helmy, N., EL-Gushi, A. (2020). Detection of Brucella organisms from Egyptian raw milk using cultural and molecular techniques. EKB Journal Management System, 18(2), 14-19. doi: 10.21608/kvmj.2020.39842.1007
Ibrahim Aman; Ibrahim AL-Hawary; Nashwa Helmy; Ahmed EL-Gushi. "Detection of Brucella organisms from Egyptian raw milk using cultural and molecular techniques". EKB Journal Management System, 18, 2, 2020, 14-19. doi: 10.21608/kvmj.2020.39842.1007
Aman, I., AL-Hawary, I., Helmy, N., EL-Gushi, A. (2020). 'Detection of Brucella organisms from Egyptian raw milk using cultural and molecular techniques', EKB Journal Management System, 18(2), pp. 14-19. doi: 10.21608/kvmj.2020.39842.1007
Aman, I., AL-Hawary, I., Helmy, N., EL-Gushi, A. Detection of Brucella organisms from Egyptian raw milk using cultural and molecular techniques. EKB Journal Management System, 2020; 18(2): 14-19. doi: 10.21608/kvmj.2020.39842.1007

Detection of Brucella organisms from Egyptian raw milk using cultural and molecular techniques

Kafrelsheikh Veterinary Medical Journal
Article 4, Volume 18, Issue 2, October 2020, Page 14-19  XML PDF (298.71 K)
Document Type: Original Article
DOI: 10.21608/kvmj.2020.39842.1007
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Authors
Ibrahim Aman email 1; Ibrahim AL-Hawary2; Nashwa Helmy3; Ahmed EL-Gushi3
1Department of Food Hygiene, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt
2Department of Fish Processing and Biotechnology, Dean of Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Egypt
3Animal Health Research Institute, El-Doki, Egypt
Abstract
Aim: This study aimed to detect Brucella organisms in Egyptian raw milk using cultural and molecular techniques.
Methods: Two hundred bulk milk samples were collected from 2 cities at El-Gharbia and Kafer El-Sheikh Governorates during the summer and winter seasons. All samples were examined by the traditional cultural method and real-time polymerase chain reaction (qRT-PCR). All positive isolates were identified by biochemical tests and serotyping using monospecific antisera also examined by multiplex PCR primers for more identification.
Results: Samples collected in summer seasons from the two cities showed no colonies characteristic to Brucella organisms while in winter season five samples showed positive characteristic features of Brucella organisms after culturing on Brucella specific media. Using qRT-PCR, 43 samples (13 in summer and 30 in winter) were positive for Brucella organisms. All positive RT-PCR samples and isolated strains were examined for the detection of the virulence genes Ure, bvfA and virB using conventional PCR. Ure, bvfA and virB were detected in 88%, 79% and 74% of milk samples and 100%, 80% 100% of isolates, respectively.
Conclusion: These results suggested that the use of the specific PCR assay is a rapid, reliable, and accurate technique in comparison to traditional methods for the detection of Brucella species in milk samples.
 
Keywords
Milk; Brucella; Culture; PCR; Virulence genes
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