Ameliorative effects of Pergamum harmala seed extract on obese diabetic rats | ||||
| Mansoura Veterinary Medical Journal | ||||
| Article 10, Volume 21, Issue 3, September 2020, Page 116-120 PDF (984.83 K) | ||||
| Document Type: Original Articles | ||||
| DOI: 10.21608/mvmj.2020.21.319 | ||||
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| Authors | ||||
| Neveen Magdy1; Mohamed Salama1; Youssef Alsaedy2; Gehad El-Sayed1 | ||||
| 1Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. | ||||
| 2Department of Physiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. | ||||
| Abstract | ||||
| Objective:To explore the potential antidiabetic activity of methanolic extract of Harmal seeds in obese-diabetic rats. Design: Randomized controlled experimental study. Animals: Forty male Sprague Dawley rats. Procedures: The P. harmala seeds methanolic extract was prepared and orally administered at two doses of 150 and 250 mg/kg to two groups of streptozotocin-induced diabetic rats. Two additional control groups were used as healthy control and obese-diabetic control groups. Animals were euthanized after 8 weeks of experimental period, blood and tissue samples were collected. Liver tissue samples were used to determine antioxidant and oxidative stress markers; while those from adipose tissue were used for estimation of PPAR gamma expression. Results:Supplementation of P. harmala methanolic extract with both doses (150 and 250 mg/kg) to diabetic rats (G3 and G4) significantly reversed the observed alterations in the levels of blood glucose, cholesterol, triglyceride, LDL, malondialdehyde (MDA), reduced glutathione (GSH), and superoxide dismutase (SOD) (P <0.05). . In addition, the downregulation of PPAR gamma expression in diabetic rats (G2) was also restored in rats (G3 and G4) supplemented with P.harmala methanolic extract. Conclusion and clinical relevance: Our finding revealed that Harmal seed extract has a potent antidiabetic activity in streptozotocin-induced diabetic rats that can be used as a dietary supplement by diabetic patients. | ||||
| Keywords | ||||
| Obesity; Type 2 Diabetes; Methanolic extract of P. harmala seed | ||||
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