Synthesis, Characterization and In vitro Antimicrobial Activity of Florfenicol-Chitosan Nanocomposite
Youssef, F., Mohamed, G., Ismail, S., Elzorba, H., Galal, A., Elbanna, H. (2021). Synthesis, Characterization and In vitro Antimicrobial Activity of Florfenicol-Chitosan Nanocomposite. EKB Journal Management System, 64(2), 941-948. doi: 10.21608/ejchem.2020.43238.2883
Fady Youssef; Gehad Mohamed; Sameh Ismail; Hisham Elzorba; Ahmed Galal; Hossny Elbanna. "Synthesis, Characterization and In vitro Antimicrobial Activity of Florfenicol-Chitosan Nanocomposite". EKB Journal Management System, 64, 2, 2021, 941-948. doi: 10.21608/ejchem.2020.43238.2883
Youssef, F., Mohamed, G., Ismail, S., Elzorba, H., Galal, A., Elbanna, H. (2021). 'Synthesis, Characterization and In vitro Antimicrobial Activity of Florfenicol-Chitosan Nanocomposite', EKB Journal Management System, 64(2), pp. 941-948. doi: 10.21608/ejchem.2020.43238.2883
Youssef, F., Mohamed, G., Ismail, S., Elzorba, H., Galal, A., Elbanna, H. Synthesis, Characterization and In vitro Antimicrobial Activity of Florfenicol-Chitosan Nanocomposite. EKB Journal Management System, 2021; 64(2): 941-948. doi: 10.21608/ejchem.2020.43238.2883

Synthesis, Characterization and In vitro Antimicrobial Activity of Florfenicol-Chitosan Nanocomposite

Egyptian Journal of Chemistry
Article 35, Volume 64, Issue 2, February 2021, Page 941-948  XML PDF (629.81 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2020.43238.2883
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Authors
Fady Youssef1; Gehad Mohamed email 2; Sameh Ismail2; Hisham Elzorba1; Ahmed Galal1; Hossny Elbanna1
1Pharmacology Department, Faculty of Veterinary Medicine, Cairo University, 1221 Giza, Egypt
2Chemistry Department, Faculty of Science, Cairo University, 12613 Giza, Egypt
Abstract
In this paper, rapid, easy and cheap sonochemical method was described for synthesis of florfenicol-chitosan nanocomposite and evaluating its anti-bacterial effect against Escherichia coli (ATCC35218), Salmonella typhymurium (ATCC14028) and Staph. aureus (ATCC29213). Florfenicol-chitosan nanocomposite was full characterized for index, identification and morphology properties. Characterization results showed zeta sizing of florfenicol was 30.6 nm, while florfenicol-chitosan nanocomposite was 67.22 nm with zeta potential -14.5 and -28, respectively. Brunner-Emmett-Teller theory (BET) surface area was found to be 13.3, 73.2 and 103.69 m2/g for florfenicol, chitosan nanoparticles and florfenicol-chitosan nanocomposite, respectively. Raman charts confirmed the formation of florfenicol-chitosan nanocomposite without any contamination. Transmission electron microscope (TEM), scanning electron microscope (SEM) and atomic force microscope (AFM) images and data illustrated spherical to sub spherical shape of florfenicol nanoparticles on sheet shape of chitosan with size less than 75 nm. Remarkable results of florfenicol-chitosan nanocomposite as anti-bacterial agent illustrated the power of nanotechnology. However, antimicrobial activity was screened where the zone of inhibitions caused by the prepared nanocomposite were 24.7 mm, 30.6 mm and 29.3 mm compared to 17.7 mm, 16 mm and 18.7 mm of the native drug against E. coli, Salmonella typhymurium and Staphylococcus aureus, respectively.
Keywords
Florfenicol; chitosan nanoparticles; Florfenicol-chitosan nanocomposite; antimicrobial activity, Microscopic techniques
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