The Effect of Mineral Trioxide Aggregate and Other Endodontic Materials in Contact with Osteoblast-Like Cell
Aljefri, A., Alghamdi, R., Albakri, R., Yousef, R., Alzahrani, W., Nawawi, A., Hafiz, R., Almaktoom, I. (2017). The Effect of Mineral Trioxide Aggregate and Other Endodontic Materials in Contact with Osteoblast-Like Cell. EKB Journal Management System, 69(5), 2546-2551. doi: 10.12816/0041708
Abrar Aqeel Aljefri; R‏Anad Abdulkareem Alghamdi; Rahaf Abdullah Albakri; Rahaf Essam O Yousef; Wejdan Ibrahim Y Alzahrani; A‏hmed Zaki A Nawawi; Raghdan Adel A Hafiz; Ibrahem Turki Almaktoom. "The Effect of Mineral Trioxide Aggregate and Other Endodontic Materials in Contact with Osteoblast-Like Cell". EKB Journal Management System, 69, 5, 2017, 2546-2551. doi: 10.12816/0041708
Aljefri, A., Alghamdi, R., Albakri, R., Yousef, R., Alzahrani, W., Nawawi, A., Hafiz, R., Almaktoom, I. (2017). 'The Effect of Mineral Trioxide Aggregate and Other Endodontic Materials in Contact with Osteoblast-Like Cell', EKB Journal Management System, 69(5), pp. 2546-2551. doi: 10.12816/0041708
Aljefri, A., Alghamdi, R., Albakri, R., Yousef, R., Alzahrani, W., Nawawi, A., Hafiz, R., Almaktoom, I. The Effect of Mineral Trioxide Aggregate and Other Endodontic Materials in Contact with Osteoblast-Like Cell. EKB Journal Management System, 2017; 69(5): 2546-2551. doi: 10.12816/0041708

The Effect of Mineral Trioxide Aggregate and Other Endodontic Materials in Contact with Osteoblast-Like Cell

The Egyptian Journal of Hospital Medicine
Article 30, Volume 69, Issue 5, October 2017, Page 2546-2551  XML PDF (405.3 K)
Document Type: Original Article
DOI: 10.12816/0041708
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Authors
Abrar Aqeel Aljefri email 1; R‏Anad Abdulkareem Alghamdi1; Rahaf Abdullah Albakri1; Rahaf Essam O Yousef1; Wejdan Ibrahim Y Alzahrani1; A‏hmed Zaki A Nawawi2; Raghdan Adel A Hafiz2; Ibrahem Turki Almaktoom3
1Ibnsina National college
2Modern Sciences and Arts University (MSA)
3King Abdulaziz university
Abstract
Objective: The objective of the study was to compare the effect of mineral trioxide aggregate and other endodontic materials in contact with osteoblast-like cell over a time period of 20 days.
Materials and methods: Human PDL cells and osteoblasts were gathered, cultured and permitted to standardized protocols. The cell populations were characterized with the conforming surface markers following standardized processes. The specimens were produced with special concern to constant dimensions and volume in the different groups. Cell attachment and proliferation were assessed morphologically after Richardson staining and cell count was performed after 1d, 7d, 14d and 21d. All trials were done in triplets. The outcomes were statistically analyzed using the ANOVA- and Tukey-test (p < 0.05).
Results: Morphological examination showed good proliferation and cell attachment in both cements. A remarkable outcome was the prepared increasing and parallel alignment of the PDL cells in connection with MTA and particularly Biodentine. From 7d forward Biodentine indicated the highest quantity of PDL cells (p < 0.05). Biodentine and MTA caused in a significantly greater cell density in osteoblast and PDL cell culture. The other groups presented a lower PDL cell density from 7d and a lower osteoblast cell density from 14d when matched to control and cement samples (p < 0.05).
Conclusions: MTA and Biodentine showed a good biocompatibility in contact with the human osteoblasts and cells of the periodontal ligament. Regarding cell survival and proliferation particularly of PDL cells Biodentine showed good results and can be considered as a well-tolerated bioactive endodontic material.
 
Keywords
composite resin; Mineral trioxide aggregate; Biodentine; Periodontal ligament cells; Osteoblasts
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