Efficacy of olibanum and propolis medicinal extracts versus metronidazole in Giardia lamblia experimentally infected mice

Al-Ghandour, Asmaa M. Farouk; Ahmed, Hytham K.; Salem, Amal E.; Tealeb, Al-Sayed M.; Mohamed, Rasha M. S. M.; Yousef, Asmaa Mohammed

doi:10.21608/mid.2020.47108.1075

	Efficacy of olibanum and propolis medicinal extracts versus metronidazole in Giardia lamblia experimentally infected mice
Microbes and Infectious Diseases
Article 18, Volume 1, Issue 3, November 2020, Page 209-220 PDF (627.88 K)
Document Type: Original Article
DOI: 10.21608/mid.2020.47108.1075
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Authors
Asmaa M. Farouk Al-Ghandour ¹; Hytham K. Ahmed²; Amal E. Salem ³; Al-Sayed M. Tealeb⁴; Rasha M. S. M. Mohamed³; Asmaa Mohammed Yousef¹
¹Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Egypt.
²Department of Clinical Pathology, Faculty of Medicine, Zagazig University, Egypt.
³Department of Clinical Pharmacology, Faculty of Medicine, Zagazig University, Egypt.
⁴Department of pathology, Faculty of Medicine, Al-Azhar University, Egypt
Abstract
Giardiasis is a common intestinal infection, recently included by the World Health Organization in the ‘Neglected Diseases Initiative’. Despite the efficacy of nitroimidazoles; the main antigiardial chemotherapeutics, adverse effects and resistance enforced developing non-chemical alternatives. The present study aimed to assess the therapeutic efficacy of ethanol extract of olibanum (OL), propolis (PR), and their combination versus metronidazole (MTZ) against G. lamblia infection. Sixty Swiss male albino mice were randomly divided into 6 groups; 10 mice each,: Group I: normal control (non-treated; non-infected). Group II: infected with G. lamblia cysts, non-treated. On the 6th day postinfection (dPI), the remaining 4 infected groups were treated orally with: Group III: (OL). Group IV: (PR). Group V: combination of (OL+PR). Group VI: (MTZ). These mice were subjected to direct parasitological diagnosis of Giardia trophozoite in intestinal exudate, immunochromatographic test for antigen detection and histopathological studies. After 7 days therapy, complete clearance of Giardia trophozoites were in the combination of (OL+PR) and MTZ groups therapy. Lower percentages of reduction (91%) & (83%) were recorded in PR and OL-treated groups, respectively. Histopathological examination showed marked healing of intestinal mucosa using non-chemical combination and different degrees of dysplasia using MTZ, while partial healing was observed using olibanum and propolis separately. Olibanum, propolis and their combination were proved to enhance the clearance of Giardia trophozoites; with progressive improvement of the histopathological changes of jejunal mucosa, making good non-chemical alternative antigiardial therapeutics sidestepping the obstacles of metronidazole like dysplasia and teratogenicity.
Keywords
Keywords: G. lamblia; olibanum; propolis; Histopathology; immunochromatography


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