Biosynthesis and Bio-sensing Applications of Silver and Gold Metal Nanoparticles | ||||
| Egyptian Journal of Chemistry | ||||
| Article 47, Volume 64, Issue 2, February 2021, Page 1057-1063 PDF (514.95 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2020.43379.2878 | ||||
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| Authors | ||||
Mohamed Abdelaziz   1; Ahmed Shalabi 2; Abdlatef A. Radwan 3; Elmorsy Khaled 4; Rabeay Y. A. Hassan5
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| 1National research Center | ||||
| 2Department of Microbial Chemistry, Genetic Engineering and Biotechnology Division, National Research Centre, Egypt | ||||
| 3National Research Centre | ||||
| 4Anal Chem. Lab, NRC | ||||
| 5Microanalysis Laboratory, Applied Organic Chemistry Department, National Research Centre, El Bohouth St., Dokki, 12622 Giza, Egypt | ||||
| Abstract | ||||
| Biogenic nanoparticles have gained major interest because of their eco-friendly approach, as non-toxic chemicals are involved in the biosynthesis, and the preparation process carried out at ambient pressure and temperature. In the present study, two fungal strains (MM1and MM2) showed the ability to biosynthesize silver and gold nanoparticles, respectively, were isolated from local Egyptian soil samples. These two fungi were identified by the molecular technique 18srRNA as Aspergillus terreus isolate MM1-EGY and Aspergillus flavus isolate MM2-EGY. The biosynthesized silver nanoparticles by Aspergillus terreus isolate MM1-EGY exhibited UV absorbance around 420 nm and particle size range of 15-35 nm as measured by transmission electron microscopy (TEM). On the other hand, gold nanoparticles biosynthesized by Aspergillus flavus isolate MM2-EGY showed maximum UV/Vis absorbance around 550 nm and size range of 20-50 nm. The XRD of both AgNPs and AuNPS was constructed. The green synthesized gold nanoparticles were tested for sensitive and selective simultaneous voltammetric determination of neurotransmitter metabolite, vanillylmandelic acid (VMA) and homovanillic acids (HVA), while silver nanoparticles were applied for the fabrication of disposable screen-printed Ag/AgCl planner reference electrode. | ||||
| Keywords | ||||
| Biosynthesis; silver Nanoparticles; gold nanoparticles; fungi; XRD; SEM; biosensor | ||||
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