Molecular Screening of Certain Virulence Encoding Genes Associated with E. coli Strains Isolated from Diarrheic Calves
Tarabees, R., Younis, G., Hamdy, H. (2020). Molecular Screening of Certain Virulence Encoding Genes Associated with E. coli Strains Isolated from Diarrheic Calves. EKB Journal Management System, 2(2), 80-88. doi: 10.21608/jcvr.2020.121530
Reda Tarabees; Gamal Younis; Hepa Hamdy. "Molecular Screening of Certain Virulence Encoding Genes Associated with E. coli Strains Isolated from Diarrheic Calves". EKB Journal Management System, 2, 2, 2020, 80-88. doi: 10.21608/jcvr.2020.121530
Tarabees, R., Younis, G., Hamdy, H. (2020). 'Molecular Screening of Certain Virulence Encoding Genes Associated with E. coli Strains Isolated from Diarrheic Calves', EKB Journal Management System, 2(2), pp. 80-88. doi: 10.21608/jcvr.2020.121530
Tarabees, R., Younis, G., Hamdy, H. Molecular Screening of Certain Virulence Encoding Genes Associated with E. coli Strains Isolated from Diarrheic Calves. EKB Journal Management System, 2020; 2(2): 80-88. doi: 10.21608/jcvr.2020.121530

Molecular Screening of Certain Virulence Encoding Genes Associated with E. coli Strains Isolated from Diarrheic Calves

Journal of Current Veterinary Research
Article 10, Volume 2, Issue 2, October 2020, Page 80-88  XML PDF (288.62 K)
Document Type: Original Article
DOI: 10.21608/jcvr.2020.121530
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Authors
Reda Tarabeesorcid 1; Gamal Younis2; Hepa Hamdy email 3
1Department of Bacteriology, Mycology and Immunology Faculty of Veterinary Medicine, University of Sadat City, Minufiya, Egypt.
2Department of Bacteriology, Mycology and Immunology Faculty of Veterinary Medicine, Mansuora University, Egypt
3Directorie of veterinary Medicine, Almeria, Alexandria, Egyp
Abstract
Calf diarrhea is main causes of mortality among neonatal calves in large-scale cattle operations. The disease syndrome usually associated with severe economic losses. Bacteria represent the common causes of diarrhea in calves. In this study, we are collected one hundred fecal samples from diarrheic mixed-sex neonatal calves aged 1-60 days located at El-Behera Governorate were examined the presence of E. coli by bacteriological tests and further screened for the presence of some virulence encoding genes. Results were showed that 95% were positive for E. coli. The isolates belonged to 9 different serogroups namely O1, O27, O126, O119, O158, O146, O25, O148, and O115. The confirmed isolates were further examining for the existence ofsome virulence encoding genes (stx1, stx2, eaeA, and hlyA). The results appeared that,stx1, stx2, eaeA, and hylA were successfully amplified in 66.6 %,41, 6 %,16.6 %, and 16.6% of the examined isolates, respectively. In conclusion; the comprehensive understanding of the virulence encoding determinants and the subcellular mechanism of E. coli pathogenesis will help develop accurate preventive and curative measures to decrease E. coli induced calf diarrhea in large-scale cattle farms.

Keywords
E.coli; Virulence genes; Diarrhea
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