Purification and Cloning of Lipase from Micro-Organism | ||||
| Journal of Current Veterinary Research | ||||
| Article 11, Volume 2, Issue 2, October 2020, Page 89-97 PDF (458.28 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jcvr.2020.121533 | ||||
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| Authors | ||||
Heba Elgendy  ; Hanan Hassan; Atef Mohamed
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| Department of microbial biotechnology, Engineering and Biotechnology Research Institute, university of Sadat City, Egypt. | ||||
| Abstract | ||||
| Lipases are a class of serine hydrolase, which belongs to the α/β hydrolases super family. Triacylglycerol acyl esters hydrolase are carboxyl esterase that catalyses both hydrolysis and synthesis of ester formed from glycerol. Lipase enzyme that catalyzes the hydrolysis of stored triacylglycerol releasing di and monoglycerol. The primary goals of this study were to isolate and identify a lipase producing bacterium from soil sample from Sadat by 16SrRNA sequence analysis, find the sequence of lipase gene by degenerate PCR and inverse PCR and analysis of lipase gene for some important feature like active site residues. On this study anew lipolytic strain was isolated, on measurement of biochemical test information, the strain was identified as Bacillus sp BM1. The maximum zone of hydrolysis (9.8mn) and specific lipase activity (86.15U/mg) was noticed for crude lipase production by the strain. The optimum temperature and pH for the lipase were found to 45 C and pH 7, respectively. | ||||
| Keywords | ||||
| Lipase; Purification; cloning; micro-organisms | ||||
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