EVALUATION OF THE ANTICARIOGENIC EFFECT OF NANO SILVER FLUORIDE ON DEMINERALIZATION OF ENAMEL IN PRIMARY TEETH (AN IN VITRO STUDY) | ||||
| Alexandria Dental Journal | ||||
| Article 1, Volume 46, Issue 2, August 2021, Page 153-159 PDF (1.03 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/adjalexu.2020.20537.1017 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Dina El-Desouky  1; Azza Hanno1; Karin Dowidar1; Sara A. Hamza2; Lubna M. El-Desouky3
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| 1pediatric dentistry and dental public health department, faculty of dentistry, alexandria university, alexandria, egypt | ||||
| 2oral biology department, faculty of dentistry, alexandria university, alexandria, egypt | ||||
| 3pharmaceutics department, faculty of pharmacy, alexandria university, alexandria, egypt | ||||
| Abstract | ||||
| Introduction: Fluoride varnishes have proven to prevent dental decay. Alternative prepa-rations that provide further benefits may be of interest to investigate. Aim of the study: was to compare the anticariogenic effect of Nano Silver Fluoride (NSF) with fluoride varnish (FV) on primary teeth enamel. Materials and method: Forty eight sound primary molars were coated with nail varnish, leaving squares of 4x4 mm then sectioned longitudinally in a buccolingual direction into two equal halves. One half received a treatment and the other half remained untreated to serve as control. Each half was considered as a specimen (96 specimens). The NSF group (n=48) was subdivided into subgroup Ia (n=24) treated with NSF and subgroup Ib (n=24) served as negative control, the FV group (n=48) was subdivided into subgroup IIa (n=24) treated with FV and subgroup IIb (n=24) served as negative control. Specimens were subjected to pH cycling then they were examined with Vickers Microhardness device and Polarized Light Microscope. Results: Percent difference in surface microhardness of subgroup Ia and subgroup Ib was 3.79±2.13, while the percent difference between subgroups IIa and IIb was 5.40±3.79. Using Mann Whitney U test, there was no statistically significant difference between the two values (P=0.81). The percent differences in lesion depth for subgroup Ia in comparison to Ib, and IIa in comparison to IIb were -36.36±9.54 and -37.30±16.67 respectively. The difference between both values was non-significant (P=0.86). Conclusion: NSF and FV are equally effective as anticariogenic materials that can limit enamel demineralization caused by cariogenic challenge. | ||||
| Keywords | ||||
| Silver compounds; Tooth demineralization; fluoride; primary teeth | ||||
| References | ||||
|
1. Petersen PE. The World Oral Health Report 2003. World Heal Organ. 2003;1–38.
2. Dean JA, Jones JE, Vinson LAW, McDonald RE. McDonald and Avery’s dentistry for the child and adolescent. 10th ed. Dean J, editor. Elsevier Ltd; 2016.
3. Dos Santos VE, Filho AV, Ribeiro Targino AG, Pelagio Flores MA, Galembeck A, Caldas AF, et al. A new “silver-Bullet” to treat caries in children - Nano Silver Fluoride: A randomised clinical trial. J Dent. 2014;42:945–51.
4. Fejerskov O, Kidd E. Dental caries: the disease and its clinical management. 2nd ed. Fejerskov O, Kidd E, editors. blackwell munksgaard; 2008.
5. O’Mullane DM, Baez2 RJ, Jones S, Lennon MA, Petersen PE, RuggGunn AJ, et al. fluoride and oral health. Community Dent Health. 2016;33:69–99.
6. American-Dental-Association. Professionally applied topical fluoride: Evidence-based clinical recommendations. J Am Dent Assoc. 2006;137:1151–9.
7. Øgaard B, Seppä L, Rolla G. Professional Topical Fluoride Applications— Clinical Efficacy and Mechanism of Action. Adv Dent Res. 1994;8:190–201.
8. Marinho VC, Worthington H V, Walsh T, Clarkson JE. Fluoride varnishes for preventing dental caries in children and adolescents. Cochrane Database Syst Rev. 2013 Jul 11; CD002279.
9. Burns J, Hollands K. Nano Silver Fluoride for preventing caries. Evid Based Dent. 2015;16:8–9.
10. Santos L de M, Reis JIL dos, Medeiros MP de, Ramos SM, Araújo JM de. In vitro evaluation of fluoride products in the development of carious lesions in deciduous teeth. Braz Oral Res. 2009;23:296–301.
11. Yu D-G, Kimura Y, Fujita A, Hossain M, Kinoshita J-I, Suzuki N, et al. Study on Acid Resistance of Human Dental Enamel and Dentin Irradiated by Semiconductor Laser with Ag(NH 3 ) 2 F Solution . J Clin Laser Med Surg. 2002;19:141–6.
12. Delbem ACB, Bergamaschi M, Sassaki KT, Cunha RF. Effect of fluoridated varnish and silver diamine fluoride solution on enamel demineralization: pH-cycling study. J Appl Oral Sci. 2006;14:88–92.
13. Rosenblatt A, Stamford TCM, Niederman R. Silver diamine fluoride: A caries “silver-fluoride bullet.” J Dent Res. 2009;88:116–25.
14. Targino AGR, Flores MAP, Dos Santos VE, De Godoy Bené Bezerra F, De Luna Freire H, Galembeck A, et al. An innovative approach to treating dental decay in children. A new anti-caries agent. J Mater Sci Mater Med. 2014;25:2041–7.
15. Nozari A, Ajami S, Rafiei A, Niazi E. Impact of nano hydroxyapatite, nano silver fluoride and sodium fluoride varnish on primary enamel remineralization: An in vitro study. J Clin Diagnostic Res. 2017;11:ZC97–100.
16. Killeen PR. An Alternative to Null-Hypothesis Significance Tests. Psychol Sci. 2005;16:345.
17. Daniel WW. Biostatistics : A foundation for analysis in the health sciences.10th ed. John Wiley & Sons; 2013
18. Pannucci CJ, Wilkins EG. Identifying and Avoiding Bias in Research. Plast Reconstr Surg. 2010;126:619.
19. Faul F, Erdfelder E, Lang A-G, Buchner A. G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods. 2007 May;39:175–91.
20. De Gauw JH, Costa LMM, Silva RN, Santos NB, Tenorio MDH. Evaluation of the Effect of Ferrous Sulfate on Enamel Demineralization of Human Deciduous Teeth: an in Vitro Study. Rev Bahiana Odontol. 2017;8: 70-76.
21. Mohammadi N, MH Farahmand Far. Effect of fluoridated varnish and silver diamine fluoride on enamel demineralization resistance in primary dentition Najmeh. J Indian Soc Pedod Prev Dent. 2018;36:257–61.
22. Duggal M, Toumba K, Amaechi B, Kawash M, Higham S. Enamel demineralization in situ with various frequencies of carbohydrate consumption with and without fluoride toothpaste. J Dent Res. 2001;80:1721–4.
23. Teixeira JA, Costa E Silva AV, Dos Santos VE, De Melo PC, Arnaud M, Lima MG, et al. Effects of a New Nano-Silver Fluoride-Containing Dentifrice on Demineralization of Enamel and Streptococcus mutans Adhesion and Acidogenicity. Int J Dent. 2018;2018.
24. Wei D, Sun W, Qian W, Ye Y, Ma X. The synthesis of chitosan-based silver nanoparticles and their antibacterial activity. Carbohydr Res. 2009;344:2375–82.
25. Agnihotri S, Mukherji S, Mukherji S. Size-controlled silver nanoparticles synthesized over the range 5-100 nm using the same protocol and their antibacterial efficacy. RSC Adv. 2014;4:3974–83.
26. Vieira AE, Delbem AC, Sassaki KT, Rodrigues E, Cury JA, Cunha RF. Fluoride dose response in pH-cycling models using bovine enamel. Caries Res. 2005;39:514–20.
27. ten Cate JM, Duijsters PP. alternating demineralization and remineralization of artificial enamel lesions. Caries Res. 1982;16:201–10.
28. Buzalaf MAR, Hannas AR, Magalhães AC, Rios D, Honório HM, Delbem ACB. pH-cycling models for in vitro evaluation of the efficacy of fluoridated dentifrices for caries control: strengths and limitations. J Appl Oral Sci. 2010;18:316–34.
29. Cury J, Amaral R, Tenuta L, Del Bel Cury A, Tabchoury C. Low-fluoride toothpaste and deciduous enamel demineralization under biofilm accumulation and sucrose exposure. Eur J Oral Sci. 2010;118:370–5.
30. De Campos PH, Sanabe ME, Rodrigues JA, Duarte DA, Santos MTBR, Guaré RO, et al. Different bacterial models for in vitro induction of non-cavitated enamel caries-like lesions: Microhardness and polarized light miscroscopy analyses. Microsc Res Tech. 2015 Jun;78:444–51.
31. Featherstone JDB. The science and practice of caries prevention. J Am Dent Assoc. 2000;131:887–99.
32. American Academy of Pediatric Dentistry. Fluoride Therapy. Ref Man. 2018;40:250–3.
33. Corrêa JM, Mori M, Sanches HL, Cruz AD Da, Poiate E, Poiate IAVP. Silver nanoparticles in dental biomaterials. Int J Biomater. 2015;2015(Article ID 485275).
34. Tenuta LMA, Cury JA. Fluoride: its role in dentistry. Braz Oral Res. 2010;24:9–17.
35. White DJ. The Application of in Vitro Models to Research on Demineralization and Remineralization of the Teeth. Adv Dent Res. 1995 Nov;9:175–93. | ||||
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