Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production
Barghout, B., El-Sayed, S., El-Said, E. (2020). Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production. EKB Journal Management System, 21(1), 31-35. doi: 10.21608/mvmj.2020.21.105
Basma Barghout; Shaimaa El-Sayed; El-Said El-Said. "Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production". EKB Journal Management System, 21, 1, 2020, 31-35. doi: 10.21608/mvmj.2020.21.105
Barghout, B., El-Sayed, S., El-Said, E. (2020). 'Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production', EKB Journal Management System, 21(1), pp. 31-35. doi: 10.21608/mvmj.2020.21.105
Barghout, B., El-Sayed, S., El-Said, E. Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production. EKB Journal Management System, 2020; 21(1): 31-35. doi: 10.21608/mvmj.2020.21.105

Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production

Mansoura Veterinary Medical Journal
Article 4, Volume 21, Issue 1, March 2020, Page 31-35  XML PDF (638.75 K)
Document Type: Original Articles
DOI: 10.21608/mvmj.2020.21.105
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Authors
Basma Barghout; Shaimaa El-Sayed; El-Said El-Said
Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, 35516, Mansoura, Egypt
Abstract
Objective: Investigating the effect of green tea extract (GTE) on the testicular damage induced by cadmium chloride CdCl2 in male rats.
Design: Randomized controlled study.
Animals: 40 male Wistar rats.
Procedures: Rats were randomly divided into four groups: A) control group (each rat daily received pellet diet); B) GTE group each rat daily received pellet diet as well as 3 ml of 1.5 % w/v GTE, C) CdCl2 group each rat was I/P injected a single dose of 1 mg/kg CdCl2, then daily received pellet diet, and D) CdCl2+GTE group each rat was I/P injected a single dose of 1 mg/kg CdCl2 then daily received pellet diet as well as 3 ml of 1.5 % w/v GTE. After 30 days, blood samples were collected for hormonal assays (testosterone, FSH, and LH). In addition, both testes were collected; one of them was used for quantification of 17-beta hydroxysteroid dehydrogenase III (17β-HSDIII) gene expression using a real-time PCR. The other testis was used for determination of catalase and reduced glutathione; GSH, Nitric oxide (NO) and malondialdehyde (MDA) levels.
Results: CdCl2 decreasedserumtestosterone levels and its synthesis pathway (17β-HSDIII testicular gene expression). While antioxidants catalase and GSH were reduced, oxidants MDA were enriched in the testes of CdCl2-poisoned rats.  This CdCl2-promotedtesticular dysfunction was corrected via the administration of GTE to male rats.
Conclusion and clinical relevance: GTE could be used as a remedy for protecting against CdCl2-inducedtesticular damage in male rats.
Keywords
Cadmium; Green tea; testosterone; antioxidants; testes
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