Effect of breeding season and steroidogenic potential of granulosa cells and follicular fluid on maturation of camel oocytes in vitro | ||||
| Mansoura Veterinary Medical Journal | ||||
| Article 13, Volume 5, Issue 1, June 2003, Page 11-20 | ||||
| Document Type: Original Articles | ||||
| DOI: 10.21608/mvmj.2003.122803 | ||||
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| Abstract | ||||
| The effect of ovarian status and steroidogenic potential of granulosa cells and / or follicular fluid on the maturation of camel oocytes in vitro during rating and non-rating seasons were studied. The camel ovaries were collected immediately after slaughter. The oocytes were aspirated jromfollicles with 8-20 mm diameter for culture in matum tion medium (MM) under dt[ferent co-culture condiH.ons, MM+No GCs (No Granulosa Cells), MM+FGCs (Fresh Gmnulosa Cells}, MM+PGCs {Pre-incubated Granulosa Cells), MM+10%FF (F’ollicu.lar Fluid) and MM+FGCs+ I0%FF. The obtained results revealed that. the distibution of small follicles on ovaries was significantly (P<0.01) higher than the medium and preovulatory fol.licles. The different follicular populations with or without corpora lutea were signtficantly (P<0.01) higher during rating than nonrating sea son. During each season, the numbers of follicles were higher on ovaries w ilh than without corpora lutea. There were no significant dijferences in the numbers of medium and large atretic jiJllicles when two seasons were compared, w hereas the med ium atretic joUicles showed higher significant rate fP<0.01) than large follicles du.ring both seasons. The mean value of grad 1+2 oocy ies was significantly (P<0.01) higher than grad 3+4 oocytes only du.ring rating season, al.though the two groups were higher during rating season. than non-rating one. The highest rates of rnetaphase-JI oocytes were observed after co-culture with. FGCs duririg rating and non-rating seasons, whereas the lowest rates were observed after co-culture with PGCs or FF. Jn all. groups, the rates of metaphase-II oocytes were higher during rating season than non-rating one. The rate of nuclear maturation increased pro- gressively with the dwal.ion of co-culture reaching the highest at 36 hrs during rating secison. During non-rati11g season. the rate of nuclear maturation increased from 12 to 24 hrs and remained cons tant ur1 to 36 hrs. | ||||
| Keywords | ||||
| camel | ||||
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