SPECIFIC CARBOHYDRATE HYDROLYZING ENZYMES IN RELATION TO DIFFERENT INSECTICIDES TREATMENTS IN WHITEFLY, Bemisia tabaci | ||||
| Journal of Productivity and Development | ||||
| Article 3, Volume 25, Issue 3, July 2020, Page 297-306 PDF (432.29 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpd.2020.122867 | ||||
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| Authors | ||||
Amira Mohamed1; Hamza El-Sharkawy  2; Saied Mehana2; Hassan Salem3
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| 1Plant Production Department, Faculty of Technology and Development, Zagazig University, Zagazig, Egypt . | ||||
| 2Department of Plant Production, Faculty of Technology &Development, Zagazig University, Egypt | ||||
| 3Plant Protection Research Institute ( Branch Zagazig);Center Agric. Research, Giza, Egypt | ||||
| Abstract | ||||
| Toxicity of acetamiprid (mospilan 20% Sp), chlorpyrifos-methyl (reldan22.5% EC) and pyriproxyfen (admiral 10% Ew) against laboratory and field strains of whitefly, Bemisiatabaci was studied. Evaluation of biochemical alterations of carbohydrate hydrolyzing enzymes (amylase, trehalase and invertase)activity were recorded and discussed as biomarkers for acetamiprid, chlorpyrifos-methyl and pyriproxyfen toxicity in the both of strains of B.tabaci. Results indicated that these compounds decreased the activities of amylase and trehalase enzymes in laboratory and field strains. Regardingto invertase enzyme, acetamiprid increased the activity ofinvertase enzymein the two strains while chlorpyrifos-methyl elevated the activity of invertase enzyme in laboratory strain, pyriproxyfen reduced the activity of invertase enzyme in both strains. Field study was conducted to evaluate the efficacy of tested insecticides against the adults of B. tabacion tomato crop, during seasons 2018 and 19 at Zagazig district, Sharkia Governorate. Results showed that acetamiprid was the most effective insecticide in both seasons, which achieved 86.81 and 88.20% of reduction percentage in B. tabaci population, followed by chlorpyrifos- methyl recording77.17 and 81.82%, pyriproxyfen was the least potent compound which recorded 59.58and 63.62% of reduction percentage in the pest population in seasons 2018 and 2019 respectively. Conclusively, it was proved through this study that the neonicotined, acetamiprid was the most potent toxicant against b. tabaci, so we used acetamiprid in b. tabaci management. | ||||
| Keywords | ||||
| Whitefly; acetamiprid; chlorpyrifosmethyl; pyriproxyfen; relative activity | ||||
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