Quantification of DNA and AgNORs correlating to cyclin D1-immunoexpression in human breast cancer
Hamed, M., Mohamed, S., Youssef, E., Rashad, R., Yahia, M. (2020). Quantification of DNA and AgNORs correlating to cyclin D1-immunoexpression in human breast cancer. EKB Journal Management System, 2(4), 54-68. doi: 10.21608/jmals.2020.123510
Mahmoud Assem Hamed; Saeed Mahmoud Saeed Mohamed; Eman Ahmed Youssef; Radwa Mohammed Rashad; Mona Abdel-Hamed Yahia. "Quantification of DNA and AgNORs correlating to cyclin D1-immunoexpression in human breast cancer". EKB Journal Management System, 2, 4, 2020, 54-68. doi: 10.21608/jmals.2020.123510
Hamed, M., Mohamed, S., Youssef, E., Rashad, R., Yahia, M. (2020). 'Quantification of DNA and AgNORs correlating to cyclin D1-immunoexpression in human breast cancer', EKB Journal Management System, 2(4), pp. 54-68. doi: 10.21608/jmals.2020.123510
Hamed, M., Mohamed, S., Youssef, E., Rashad, R., Yahia, M. Quantification of DNA and AgNORs correlating to cyclin D1-immunoexpression in human breast cancer. EKB Journal Management System, 2020; 2(4): 54-68. doi: 10.21608/jmals.2020.123510

Quantification of DNA and AgNORs correlating to cyclin D1-immunoexpression in human breast cancer

Journal of Medical and Life Science
Article 1, Volume 2, Issue 4, December 2020, Page 54-68  XML PDF (1.09 MB)
Document Type: Original Article
DOI: 10.21608/jmals.2020.123510
View on SCiNiTO View on SCiNiTO
Authors
Mahmoud Assem Hamed email 1; Saeed Mahmoud Saeed Mohamed2; Eman Ahmed Youssef1; Radwa Mohammed Rashad3; Mona Abdel-Hamed Yahia1
1Department of Histochemistry and Cell Biology, Medical Research Institute, Alexandria University, Alexandria, Egypt
2Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, West Kordufan University, Kordufan, Sudan
3Department of Pathology, Medical Research Institute, Alexandria University, Alexandria, Egypt
Abstract
Background: Assessment of cell proliferation through the evaluation of cyclin D1, AgNORs, and DNA content provides a promising approach to the prognosis of breast cancer. Therefore, this work aimed to evaluate the prognostic significance of AgNORs and DNA image cytometry, and their relation to cyclin D1 immunoreactivity in breast cancer.
Subjects and methods: This study was carried out on 50 prospective biopsies taken from patients with breast tumors. They were divided into control (10 cases) and benign (10 cases) as well as malignant (30 cases) groups. Computer-assisted image analysis cytometry is applied to evaluate the count of positive reactions of AgNORs and cyclin D1, and an integrated optical density (IOD) technique is used to quantify DNA content in the tissues stained by Feulgen stain and to assess the density of cyclin D1 immunoreactivity.
Results: The comparison between the mean count of cyclin D1 positive reaction (Mean ± SD: 31.10 ± 26.39) and AgNORs dots (Mean ± SD: 4.64 ± 2.77) revealed a statistically significant difference (P <0.001) in the normal group and in the malignant group as well as in total cases. The association between the IOD parameter forFeulgen stain (Mean ± SD: 77.35 ± 27.99) and cyclin D1 immunostaining (Mean ± SD: 151.90 ± 37.38) showed a statistically significant difference throughout all of the individual groups (P <0.001).
Conclusions: These results indicate that image cytometry may be a valuable method for quantitating DNA, AgNORs, and cyclin D1-immunostaining, and that provides valuable information that may have prognostic significance for patients with breast cancer.
Keywords
DNA quantification; AgNORs; Cyclin D1; Image cytometry; Immunohistochemistry; Breast cancer
Statistics
Article View: 241
PDF Download: 275