Comparative study on the use of different solvents, and their effect on the stability of ppr attenuated vaccine | ||||
| Mansoura Veterinary Medical Journal | ||||
| Article 1, Volume 7, Issue 1, June 2005, Page 1-8 PDF (368.68 K) | ||||
| Document Type: Original Articles | ||||
| DOI: 10.21608/mvmj.2005.124706 | ||||
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| Abstract | ||||
| Paste des petils ruminants (PPR) virus as one of Morbillivirus (Paramyxoviridae) is a heat labile virus. In Egypt, as a hot-country-in general we are urgently in need to find out a suitable solvent that may be act as heat tolerant. In this work, we tried to select the best solvent which provide a longer stability of reconstituted PPR vaccine six new solvents were studied in a comparative with the traditional control solvent (physiological saline solution). These solvent soilitions included physiological saline solution, 1 M magnesium sulphate solution (MgSO4). 2.5% lactalbumin hydrolysate solution. a mix-ture of lactalbumin hydrolysate 2.5% and sucrose 5% solution 5% sucrose solution. A mixture of 1 M magnesium sulphate and saline solution. and dimethyl sulfoxide 2.5% solution. By using these solvents solutions. the reconstituting PPR vaccine was kept on ice and at 37C, titration of the virus infectivity on Vero cells was carried out at 0, 1 and 2 hours post reconstitution . it was revealed that the best solvent solution was the mixture of 2.5% lactalbumin hydrolysate and 5% sucrose that could keep the loss in the original virus titer at the most minimum. | ||||
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