Molecular Identification of Thripids Attacking Olive Groves at Ismailia, Egypt
Agamy, E., El-Husseini, M., El- Sebaey, I., Wafy, M. (2017). Molecular Identification of Thripids Attacking Olive Groves at Ismailia, Egypt. EKB Journal Management System, 10(4), 43-55. doi: 10.21608/eajbsa.2017.12483
Essam A. Agamy; Monir M. El-Husseini; Iman I. El- Sebaey; Maaly E. Wafy. "Molecular Identification of Thripids Attacking Olive Groves at Ismailia, Egypt". EKB Journal Management System, 10, 4, 2017, 43-55. doi: 10.21608/eajbsa.2017.12483
Agamy, E., El-Husseini, M., El- Sebaey, I., Wafy, M. (2017). 'Molecular Identification of Thripids Attacking Olive Groves at Ismailia, Egypt', EKB Journal Management System, 10(4), pp. 43-55. doi: 10.21608/eajbsa.2017.12483
Agamy, E., El-Husseini, M., El- Sebaey, I., Wafy, M. Molecular Identification of Thripids Attacking Olive Groves at Ismailia, Egypt. EKB Journal Management System, 2017; 10(4): 43-55. doi: 10.21608/eajbsa.2017.12483

Molecular Identification of Thripids Attacking Olive Groves at Ismailia, Egypt

Egyptian Academic Journal of Biological Sciences. A, Entomology
Article 4, Volume 10, Issue 4 - Serial Number 1, June 2017, Page 43-55  XML PDF (762.75 K)
Document Type: Original Article
DOI: 10.21608/eajbsa.2017.12483
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Authors
Essam A. Agamy1; Monir M. El-Husseini1; Iman I. El- Sebaey2; Maaly E. Wafy2
1Dept. of Economic Entomology and Pesticides, Fac. of Agriculture, Cairo University, Giza, Egypt.
2Plant Protection Res. Inst. Agriculture Res. Cent., Dokki, Giza, Egypt
Abstract
A little attention has been given on molecular identification of thrips (Insecta: Thysanoptera) in Egypt. Thus, the primers ITS4A, 28S rRNA, 18S rRNA and the primer pairs COI-1 + COI-2 + COI-3 were used to identify the three dominant thrips species (Haplothrips cahirensis karny; Dendrothrips eremicola Priesener and Hydatothrips kassimanus Priesener). The samples were collected from a private olive grove during 2013 and 2014 at Ismailia governorate, Egypt. DNA sequence data for 28S rRNA gene showed apositiveresponseto H. cahirensis and D. eremicola only, while 18S rRNA reacted with all species. ITS4A and COI-1 + COI-2 + COI-3 showed a negative response to the studied species. DNA sequences of the species based on 18S rRNA were recorded in the Gene Bank. The phylogenetic tree of the three thrips species was given also.
Keywords
thrips; Thysanoptera; identification; primers; 18S rRNA
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