Expression of cathepsin L gene of fasciola gigantica in different developmental stages
(2008). Expression of cathepsin L gene of fasciola gigantica in different developmental stages. EKB Journal Management System, 10(2), 21-35. doi: 10.21608/mvmj.2008.126221
. "Expression of cathepsin L gene of fasciola gigantica in different developmental stages". EKB Journal Management System, 10, 2, 2008, 21-35. doi: 10.21608/mvmj.2008.126221
(2008). 'Expression of cathepsin L gene of fasciola gigantica in different developmental stages', EKB Journal Management System, 10(2), pp. 21-35. doi: 10.21608/mvmj.2008.126221
Expression of cathepsin L gene of fasciola gigantica in different developmental stages. EKB Journal Management System, 2008; 10(2): 21-35. doi: 10.21608/mvmj.2008.126221

Expression of cathepsin L gene of fasciola gigantica in different developmental stages

Mansoura Veterinary Medical Journal
Article 3, Volume 10, Issue 2, December 2008, Page 21-35  XML PDF (413.79 K)
Document Type: Original Articles
DOI: 10.21608/mvmj.2008.126221
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Abstract
Fascioliasis Is one of the most serious parasiiic infestations of livestock. It is caused by Fasciola hepatica (tremperate regions) and Fasciola gigantica (Tropf.cal reglons),It also has a zoonotic importance, the proteolytic enzyme: cathepsin-L cysteine proteinase (28-28,5 KDa) helps the parasite tn tissue invasion. host lmmune evasion and nutrients intake, This study aimed to check weather Fasciola gigantica flukes depend upon cathepsin-L throughout their life cycle in. snail and mammalian hosts or not, For that cuthepstn-L gene was investigated (n adult and other developmental stages of fasciola glgantica using semi-quantitative RT•PCR assay. Cathepsin-L protein was detected by using sodium dodecyl sulphate- polyacrylamide get electrophoresis [SDS-PAGE) analysts and detection of cathepsfn-L cysteine proteinase antigen in examined stages by Enzyme linked tmmunotransfer blot (EITP) analysts.
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