IMPROVEMENT OF BUFFALO EMBRYO DEVELOPMENT IN VITRO; EFFECT OF DIFFERENT CULTURE MEDIA
Mostager, A., Montaser, A., Badr, M., Hegab, A., Zaabel, S. (2017). IMPROVEMENT OF BUFFALO EMBRYO DEVELOPMENT IN VITRO; EFFECT OF DIFFERENT CULTURE MEDIA. EKB Journal Management System, 18(1), 45-50. doi: 10.21608/mvmj.2017.126864
Amira Mostager; Abd El-Moneim Montaser; Magdy Ramdan Badr; Abd El-Raouf Osman Hegab; Samy Moawad Zaabel. "IMPROVEMENT OF BUFFALO EMBRYO DEVELOPMENT IN VITRO; EFFECT OF DIFFERENT CULTURE MEDIA". EKB Journal Management System, 18, 1, 2017, 45-50. doi: 10.21608/mvmj.2017.126864
Mostager, A., Montaser, A., Badr, M., Hegab, A., Zaabel, S. (2017). 'IMPROVEMENT OF BUFFALO EMBRYO DEVELOPMENT IN VITRO; EFFECT OF DIFFERENT CULTURE MEDIA', EKB Journal Management System, 18(1), pp. 45-50. doi: 10.21608/mvmj.2017.126864
Mostager, A., Montaser, A., Badr, M., Hegab, A., Zaabel, S. IMPROVEMENT OF BUFFALO EMBRYO DEVELOPMENT IN VITRO; EFFECT OF DIFFERENT CULTURE MEDIA. EKB Journal Management System, 2017; 18(1): 45-50. doi: 10.21608/mvmj.2017.126864

IMPROVEMENT OF BUFFALO EMBRYO DEVELOPMENT IN VITRO; EFFECT OF DIFFERENT CULTURE MEDIA

Mansoura Veterinary Medical Journal
Article 18, Volume 18, Issue 1, June 2017, Page 45-50  XML PDF (457.19 K)
DOI: 10.21608/mvmj.2017.126864
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Authors
Amira Mostager* 1; Abd El-Moneim Montaser1; Magdy Ramdan Badr2; Abd El-Raouf Osman Hegab3; Samy Moawad Zaabel1
1Theriogenology department, Faculty of Veterinary Medicine, Mansoura University, Egypt
2Artificial Insemination and Embryo Transfer Department, Animal Reproduction Research Institute
3Biology Department, Faculty of Science, Taif University, KSA
Abstract

The aim of the present study to compare 4 different types of media, SOF, TCM-119, CR1aa and KSOM, for in vitro culture of buffalo embryo. This study was under taken in the Department of Artificial insemination and embryo transfer, Animal Reproduction Research Institute (ARRI), Al-Haram, Giza .The selec&#116;ed cumulus oocyte complexes(COCs) with a compact cumulus cells and evenly granulated ooplasm were in vitro matured at 38.5°C in atmosphere of 5% CO2 with high humidity .After 24h of culture, oocyte were used for in vitro fertilization . Following 18h of insemination, oocyte were randomly assigned to investigate the effect of different types of media from the zygote to day 7 post-insemination . there were no significant difference in cleavage rate among 4 media However, the morula and blastocyst stage increased significantly(P<0.05) in using SOF (26.03, 17.88% respectively) and also by using CR1aa (25.37, 16.42% respectively) compared with TCM (11.86, 5.08% respectively) and KSOM (9.62, 3.85 %respectively).There is no significant difference between SOF and CR1aa.In conclusion, the current data reveled that SOF is the best media used in embryo development in buffalo. Key words: SOF. In vitro culture media. Buffalo
Keywords
Buffalo; SOF; TCM-119; CR1aa; KSOM
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