IDENTIFICATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN EXON 2 OF BONE MORPHOGENETIC PROTEIN 15 GENE IN EGYPTIAN BUFFALOES
El-Bayomi, K., El Araby, I., Saleh, A., Osman, H., Eltarabany, M., Awad, A., Abd El-fattah, A., Abu El-Magd, M. (2017). IDENTIFICATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN EXON 2 OF BONE MORPHOGENETIC PROTEIN 15 GENE IN EGYPTIAN BUFFALOES. EKB Journal Management System, 18(1), 235-242. doi: 10.21608/mvmj.2017.127392
Khairy M El-Bayomi; Iman E El Araby; Ayman A Saleh; Hoda Z Osman; Mahmoud S Eltarabany; Ashraf Awad; Amir H Abd El-fattah; Mohammed Abu El-Magd. "IDENTIFICATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN EXON 2 OF BONE MORPHOGENETIC PROTEIN 15 GENE IN EGYPTIAN BUFFALOES". EKB Journal Management System, 18, 1, 2017, 235-242. doi: 10.21608/mvmj.2017.127392
El-Bayomi, K., El Araby, I., Saleh, A., Osman, H., Eltarabany, M., Awad, A., Abd El-fattah, A., Abu El-Magd, M. (2017). 'IDENTIFICATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN EXON 2 OF BONE MORPHOGENETIC PROTEIN 15 GENE IN EGYPTIAN BUFFALOES', EKB Journal Management System, 18(1), pp. 235-242. doi: 10.21608/mvmj.2017.127392
El-Bayomi, K., El Araby, I., Saleh, A., Osman, H., Eltarabany, M., Awad, A., Abd El-fattah, A., Abu El-Magd, M. IDENTIFICATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN EXON 2 OF BONE MORPHOGENETIC PROTEIN 15 GENE IN EGYPTIAN BUFFALOES. EKB Journal Management System, 2017; 18(1): 235-242. doi: 10.21608/mvmj.2017.127392

IDENTIFICATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN EXON 2 OF BONE MORPHOGENETIC PROTEIN 15 GENE IN EGYPTIAN BUFFALOES

Mansoura Veterinary Medical Journal
Article 27, Volume 18, Issue 1, June 2017, Page 235-242  XML PDF (1.41 MB)
DOI: 10.21608/mvmj.2017.127392
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Authors
Khairy M El-Bayomi1; Iman E El Araby* 1; Ayman A Saleh1; Hoda Z Osman2; Mahmoud S Eltarabany1; Ashraf Awad1; Amir H Abd El-fattah1; Mohammed Abu El-Magd3
1Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Egypt
2Animal Production Research Institute (APRI), Agricultural Research Centre, Ministry of Agriculture, Egypt
3Department of Anatomy & Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University.
Abstract
Bone Morphogenetic Protein 15 (BMP 15) is a member of transforming growth factor-β (TGF- β) superfamily which plays an important role in ovarian follicular development and ovulation rate. In this study a partial sequence of this gene was screened for polymorphisms. PCR amplification of DNA isolated from blood of 100 Egyptian buffaloes hiefers (50 infertile and 50 normal). Revealed two loci, BMP15.1 (222 bp) and BMP15.2 (222 bp) which includes exon 2 of BMP 15 gene. Polymorphisms in these two loci were detected by using direct sequencing and single strand conformational polymorphism (SSCP) technique. A single pattern of SSCP was detected. This was confirmed by nucleotide sequencing which revealed absence of single nucleotides polymorphisms (SNPs) or any other type of polymorphisms in the two loci. The sequencing and PCR-SSCP patterns revealed no polymorphism in all studied animals. Further studies must be done on another loci on BMP 15 gene or other genes related to infertility in Egyptian buffaloes
Keywords
Buffalo; Egypt; Infertility; BMP15
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