Optimization and validation of spectrophotometric determination of glucosamine in dosage Form with sodium 1,2-naphthoquinone-4-sulphonate
Mahmoud, W., El-Gindy, A., Eid, B., Neamatallah, T., Hadad, G. (2018). Optimization and validation of spectrophotometric determination of glucosamine in dosage Form with sodium 1,2-naphthoquinone-4-sulphonate. EKB Journal Management System, 2(1), 47-53. doi: 10.21608/rpbs.2018.12825
Waleed Mahmoud; Alaa El-Gindy; Basma G. Eid; Thikrayat Neamatallah; Ghada Hadad. "Optimization and validation of spectrophotometric determination of glucosamine in dosage Form with sodium 1,2-naphthoquinone-4-sulphonate". EKB Journal Management System, 2, 1, 2018, 47-53. doi: 10.21608/rpbs.2018.12825
Mahmoud, W., El-Gindy, A., Eid, B., Neamatallah, T., Hadad, G. (2018). 'Optimization and validation of spectrophotometric determination of glucosamine in dosage Form with sodium 1,2-naphthoquinone-4-sulphonate', EKB Journal Management System, 2(1), pp. 47-53. doi: 10.21608/rpbs.2018.12825
Mahmoud, W., El-Gindy, A., Eid, B., Neamatallah, T., Hadad, G. Optimization and validation of spectrophotometric determination of glucosamine in dosage Form with sodium 1,2-naphthoquinone-4-sulphonate. EKB Journal Management System, 2018; 2(1): 47-53. doi: 10.21608/rpbs.2018.12825

Optimization and validation of spectrophotometric determination of glucosamine in dosage Form with sodium 1,2-naphthoquinone-4-sulphonate

Records of Pharmaceutical and Biomedical Sciences
Article 6, Volume 2, Issue 1, March 2018, Page 47-53  XML PDF (578.49 K)
Document Type: Original Article
DOI: 10.21608/rpbs.2018.12825
View on SCiNiTO View on SCiNiTO
Authors
Waleed Mahmoud1, 2; Alaa El-Gindy1; Basma G. Eid3; Thikrayat Neamatallah3; Ghada Hadad email orcid 1
1Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt
2Sustainable Chemistry and Material Resources, Institute of Sustainable and Environmental Chemistry, Leuphana University Lüneburg, C13, DE-21335 Lüneburg, Germany
3Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia
Abstract
A rapid, sensitive and simple optimized spectrophotometric method is developed for the determination of Glucosamine (GL) based on reaction of GL with sodium 1,2-naphthoquinone-4-sulphonate (NQS) in alkaline medium forming orange colored product which measured at 451 nm. The method optimization was essential as many procedural parameters influenced the efficiency and reproducibility of the derivatization process including the reaction time, reaction temperature,NQS concentration, alkali concentration and solvents. The objective of our study was to optimize and validate a spectrophotometric method using the reaction of GL with NQS for determining GL in dosage form. The factors that may influence the efficiency and reproducibility of the derivatization process were identified and optimized. The proposed method was validated and successfully applied for the analysis of GL in dosage form. Statistical comparison of the results of the proposed method with the reference method showed excellent agreement and proved that no significant difference in the accuracy and precision. The developed method is easy to use, accurate and least cost-effective for routine studies relative to HPLC and other techniques
Keywords
Glucosamine; 1,2-naphthoquinone-4-sulphonate; Spectrophotometric determination; Pharmaceutical analysis
Statistics
Article View: 306
PDF Download: 378