Effect of Some Plant Extracts on The Biochemical Parameters, AChE and GST Activities of The Mosquito, Culex pipiens L. (Diptera: Culicidae)
Shahat, M., El-Sheikh, T., Hammad, K., Hasaballah, A., Shehata, A. (2020). Effect of Some Plant Extracts on The Biochemical Parameters, AChE and GST Activities of The Mosquito, Culex pipiens L. (Diptera: Culicidae). EKB Journal Management System, 12(2), 69-80. doi: 10.21608/eajbse.2020.128498
Mohamed A.M. Shahat; Tarek M.Y. El-Sheikh; Kotb M. Hammad; Ahmed I. Hasaballah; Ahmed Z.I. Shehata. "Effect of Some Plant Extracts on The Biochemical Parameters, AChE and GST Activities of The Mosquito, Culex pipiens L. (Diptera: Culicidae)". EKB Journal Management System, 12, 2, 2020, 69-80. doi: 10.21608/eajbse.2020.128498
Shahat, M., El-Sheikh, T., Hammad, K., Hasaballah, A., Shehata, A. (2020). 'Effect of Some Plant Extracts on The Biochemical Parameters, AChE and GST Activities of The Mosquito, Culex pipiens L. (Diptera: Culicidae)', EKB Journal Management System, 12(2), pp. 69-80. doi: 10.21608/eajbse.2020.128498
Shahat, M., El-Sheikh, T., Hammad, K., Hasaballah, A., Shehata, A. Effect of Some Plant Extracts on The Biochemical Parameters, AChE and GST Activities of The Mosquito, Culex pipiens L. (Diptera: Culicidae). EKB Journal Management System, 2020; 12(2): 69-80. doi: 10.21608/eajbse.2020.128498

Effect of Some Plant Extracts on The Biochemical Parameters, AChE and GST Activities of The Mosquito, Culex pipiens L. (Diptera: Culicidae)

Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology
Article 8, Volume 12, Issue 2, December 2020, Page 69-80  XML PDF (638.27 K)
Document Type: Original Article
DOI: 10.21608/eajbse.2020.128498
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Authors
Mohamed A.M. Shahat; Tarek M.Y. El-Sheikh; Kotb M. Hammad; Ahmed I. Hasaballah; Ahmed Z.I. Shehata
Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11651, Egypt.
Abstract
The present study was undertaken in order to investigate the effect of median lethal concentration (LC50) of methanol, ethyl acetate, chlorobenzene and hexane extracts from leaves of Origanum syriacum, Pergularia tomentosa, Senna italica, and Otostegia fruticose against different biological aspects of the 3rd instar larvae of Culex pipiens. The effects were examined on the biochemical composition of the larval body and the activities of acetylcholinesterase (AChE) and glutathione S-transferase (GST). Based on obtained results, hexane extract showedmore activity against 3rd instar larvae of C. pipiens followed by chlorobenzene, ethyl acetate, and methanol extract. Tested extracts caused a modification in biochemical composition. The LC50 of each treatment decreased the total carbohydrate, protein, and lipid contents as compared with the control. Tested extracts also showed a neurotoxic activity as evidenced by the inhibition effect of the AChE activity that was significantly (P<0.001) decreased. Stimulation of the detoxification system of tested larvae was evidenced by the promoted activity of GST as compared with the untreated group.In general, tested extracts used in the present study may be considered as promising control agents against the mosquito vector, C. pipiens.
Keywords
Culex pipiens; AChE; GST; Plant extracts; Carbohydrate; lipids; proteins
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