USING OF GUINEA PIGS AS ANIMAL MODEL TO DETERMINE THE PD50 OF LOCAL OIL BIVALENT FMD VACCINE | ||||
| Mansoura Veterinary Medical Journal | ||||
| Article 2, Volume 17, Issue 2, June 2016, Page 797-809 | ||||
| DOI: 10.21608/mvmj.2016.129069 | ||||
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| Author | ||||
| Eman EL- Garf* | ||||
| Veterinary Serum and Vaccine Research Institute; Abassia, Cairo | ||||
| Abstract | ||||
| An inactivated bivalent oil foot and mouth disease (FMD) vaccine was prepared using the local isolated types of the caused virus (FMDV type O/Egypt/93 and A/1/Egypt/2006). This vaccine was subjected to the quality control tests which revealed that it is free from foreign contaminants, safe and potent for Guinea pigs which were used as a model animal for determination of the vaccine PD50. The obtained results showed that vaccinated Guinea pigs exhibited good levels of homologous FMD antibodies; as shown by serum neutralization test and ELISA; by the 3rd week post vaccination enabling them to withstand the challenge viruses with protection ratios of 100%; 75% against type O for undiluted and 1/4 diluted vaccine respectively while 100% protection was detected against type A with the 2 vaccine dilutions. Higher vaccine dilutions were found to be non-protective showing low protection ratios (25-0%) It was found that Guinea pig 50% protective dose (GPPD50) was 7.94 for FMD virus type O and 11.06 for type A. Such permissible values of vaccine potency and PD50 confirm the possibility of the use of Guinea pigs to determine the PD50 of FMD vaccine as a model animal instead of cattle saving effort and cost. | ||||
| Keywords | ||||
| Guinea pigs; FMD | ||||
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