ISOLATION AND IDENTIFICATION OF DEFENSIVE FLAVONOIDS FROM BRASSICA NAPUS LEAVES EXTRACT WITH PROMISING BIOLOGICAL ACTIVITY TO CONTROL THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.)
ABAZA, A. (2018). ISOLATION AND IDENTIFICATION OF DEFENSIVE FLAVONOIDS FROM BRASSICA NAPUS LEAVES EXTRACT WITH PROMISING BIOLOGICAL ACTIVITY TO CONTROL THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.). EKB Journal Management System, 96(1), 47-64. doi: 10.21608/ejar.2018.129663
ANWAAR M. ABAZA. "ISOLATION AND IDENTIFICATION OF DEFENSIVE FLAVONOIDS FROM BRASSICA NAPUS LEAVES EXTRACT WITH PROMISING BIOLOGICAL ACTIVITY TO CONTROL THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.)". EKB Journal Management System, 96, 1, 2018, 47-64. doi: 10.21608/ejar.2018.129663
ABAZA, A. (2018). 'ISOLATION AND IDENTIFICATION OF DEFENSIVE FLAVONOIDS FROM BRASSICA NAPUS LEAVES EXTRACT WITH PROMISING BIOLOGICAL ACTIVITY TO CONTROL THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.)', EKB Journal Management System, 96(1), pp. 47-64. doi: 10.21608/ejar.2018.129663
ABAZA, A. ISOLATION AND IDENTIFICATION OF DEFENSIVE FLAVONOIDS FROM BRASSICA NAPUS LEAVES EXTRACT WITH PROMISING BIOLOGICAL ACTIVITY TO CONTROL THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.). EKB Journal Management System, 2018; 96(1): 47-64. doi: 10.21608/ejar.2018.129663

ISOLATION AND IDENTIFICATION OF DEFENSIVE FLAVONOIDS FROM BRASSICA NAPUS LEAVES EXTRACT WITH PROMISING BIOLOGICAL ACTIVITY TO CONTROL THE COTTON LEAFWORM, SPODOPTERA LITTORALIS (BOISD.)

Egyptian Journal of Agricultural Research
Article 18, Volume 96, Issue 1, March 2018, Page 47-64  XML PDF (971.22 K)
Document Type: Original Article
DOI: 10.21608/ejar.2018.129663
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Author
ANWAAR M. ABAZA
Plant Protection Research Institute,, Agricultural Research Center, Giza, Egypt
Abstract
Brassica napus plant has defensive secondary metabolites against phytophagous insects and pathogens. In extraction process, the end product contains traces of residual solvent, so, the solvent should not be non-toxic and should not interfere with the bioassay. The preliminary biological activity of ethanol, petroleum ether, methylene chloride, ethyl acetate and butanol fractions of B. napus leaves was investigated against the 4th instar larvae of Spodoptera littoralis under laboratory conditions. The ethanolic fraction showed the most toxic effect against the 4th instar larvae of S. littoralis. By phytochemical screening, B. napus leaves contain flavonoids as a major constituent. Four flavonoid compounds were isolated as crystals by column chromatographic technique using silica gel and purified on Sephadex LH-20. Their structures were elucidated as Quercetin (1), Kaempferol (2), Kaempferol-3-O-glucoside (3) and Quercetin-7-O-glucoside (4) by using UV and 1H-NMR techniques. On toxicological studies, LC50 values (0.455 and 0.091 gm/100 ml) at 7 and 14 days post treatment, respectively, showed that the toxicity of B. napus extract increased with increasing concentration and time post treatment. This indicated that B. napus extract can be used as green insecticide to control the cotton leafworm, S. littoralis. 
Keywords
Brassica napus; defensive secondary metabolites; flavonoids; column chromatograhy UV; 1H-NMR; cotton leafworm; Spdoptera littoralis
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