Preparation and characterization of silica hydrogel nanocomposites as a drug delivery system

Abdelazeem, Noha Mustafa; El-Sayed, El-Sayed M.; Abo-Naf, Sherief M.; Abu-Zeid, Hosnia M.; Meligi, Gamal A.

doi:10.21608/jsrs.2020.129910

	Preparation and characterization of silica hydrogel nanocomposites as a drug delivery system
Journal of Scientific Research in Science
Article 4, Volume 37, Part 1 (Basic Sciences), February 2020, Page 44-60 PDF (1.89 MB)
Document Type: Original Article
DOI: 10.21608/jsrs.2020.129910
View on SCiNiTO
Authors
Noha Mustafa Abdelazeem ¹; El-Sayed M. El-Sayed²; Sherief M. Abo-Naf³; Hosnia M. Abu-Zeid⁴; Gamal A. Meligi⁵
¹Physics department, Faculty of women for Arts, Science and Education, Ain Shams university, Cairo, Egypt
²Physics Department, Biophysics Branch, Faculty of Science, Ain Shams University, Cairo, Egypt.
³Glass Research Department, National Research Centre (NRC), El-Buhouth Str., Dokki, 12622 Cairo, Egypt.
⁴Physics Department, Faculty of Women for Arts; Science and Education, Ain Shams University, Cairo, Egypt.
⁵Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Abstract
Nowadays, the bacterial resistant infection risks to antibiotics have the limelight of developing new and natural antibacterial biomaterials. So, this work aimed to prepare drug carriers for Ceftriaxone (CFX) loading based on hydrogel nanocomposites for increasing the CFX loading capacity and improving the antibacterial properties of the hydrogels. Hence, a novel nanocomposites based on Ca-alginate hydrogels were prepared as antibacterial model. The morphology, textural, and the molecular interaction between the Mesoporous silica nanoparticles (MSNs) and the hydrogels were evaluated by using SEM, DCS, and FT-IR characterization techniques and loading efficiency was calculated. The loading efficiencies of (0, 5, 10 % MSNs) loaded with CFX were 2.379, 19.597 and 16.05 %, respectively. Furthermore, the antibacterial property of the hydrogels was tested by using colony forming test at three different loaded CFX concentrations. The results showed that, as MSNs % was increased in the alginate matrix, the loading CFX concentration increased. Thus, at 10% MSNs of 425.6 µg/ml CFX showed a significant viability % with (p** < 0.01) that was reached to 15.473% ± 0.399 relative to negative control. So, the hydrogels were to be used as a good CFX drug carrier and demonstrated a bactericidal effect against gram positive and gram negative bacteria.
Keywords
Antibacterial; CFX; hydrogels; MSNs; nanocomposites


References
[1] O. Andrea, I. Jancic, J. Vunduk, P. Petrovic, M. Milenkovic, B. Obradovic, "Achieving high antimicrobial activity: Composite alginate hydrogel beads releasing activated charcoal with an immobilized active agent," Carbohydrate polymers 196: (2018) 279-288.2. [2] Z. Yin, Y. Qifeng, Z. Tong, C. Xiaoqiang, W. Zhengqi, Ni Zhang, S. Yundong, C. Yong, " Antibacterial activity and mechanism of green tea polysaccharide conjugates against Escherichia coli,". Industrial Crops and Products 152: (2020) 112464. [3] L. Sanae, J. Uwingabiye, M. Frikh, L. Abdellatifi, J. Kasouati, A. Maleb, A. Bait, A. Lemnouer, M. Elouennass. "In vitro evaluation of the susceptibility of Acinetobacter baumannii isolates to antiseptics and disinfectants: comparison between clinical and environmental isolates," Antimicrobial Resistance & Infection Control 6(1): (2017) 36. [4] Z. Miao, Z. Xia, "Alginate hydrogel dressings for advanced wound management", International Journal of Biological Macromolecules 162: (2020) 14. [5] L. David, P. Durani. "Topical antimicrobial therapy of chronic wounds healing by secondary intention using iodine products," International Wound Journal 5(2): (2008) 361-368. [6] N. Fredrik, Y. Zhang, J. PK Tan, K. Xu, H. Wang, C. Yang, S. Gao et al. "Biodegradable nanostructures with selective lysis of microbial membranes." Nature chemistry 3(5): (2011) 409-414. [7] A. Zahra, G. Emtiazi, A. Zarrabi, "Novel synergistic activities of tetracycline copper oxide nanoparticles integrated into chitosan micro particles for delivery against multiple drug resistant strains: generation of reactive oxygen species (ROS) and cell death," Journal of drug delivery science and technology 44: (2018) 65-70. [8] A. Nadia, P. Couvreur, "Nanocarriers for antibiotics: a promising solution to treat intracellular bacterial infections," International journal of antimicrobial agents 43(6): (2014) 485-496. [9] M. Maria Luisa, D. Araújo Agarrayua, J. Carlosso Machado, C. Alberto Schmidt, "A fully validated microbiological assay to evaluate the potency of ceftriaxone sodium, " Brazilian Journal of Pharmaceutical Sciences 49(4): (2013) 753-762. [10] da Trindade, M. Teixeira, H. Regina Nunes Salgado, "A critical review of analytical methods for determination of ceftriaxone sodium," Critical Reviews in Analytical Chemistry 48(2): (2018) 95-101. [11] G. Cheong Hian, P. Wan Sia Heng, L. Wah Chan, "Alginates as a useful natural polymer for microencapsulation and therapeutic applications," Carbohydrate Polymers 88(1): (2012) 1-12. [12] G. Renmin, C. Li, S. Zhu, Y. Zhang, Y. Du, J. Jiang, "A novel pH-sensitive hydrogel based on dual cross-linked alginate/N-α-glutaric acid chitosan for oral delivery of protein," Carbohydrate polymers 85(4): (2011) 869-874. [13] V. Kokkarachedu, J. Tippabattini, K. Vimala, Claudio Toro, S. Emmanuel Rotimi, "Alginate-based composite materials for wound dressing application", A mini review, Carbohydrate Polymers 236: (2020) 116025. [14] M. Xiaomei, R. Li, X. Zhao, Q. Ji, Y. Xing, J. Sunarso, Y. Xia, "Biopolymer composite fibres composed of calcium alginate reinforced with nanocrystalline cellulose," Composites Part A: Applied Science and Manufacturing 96: (2017)155-163. [15] N. Valentina, S. Magnolia, M. Piludu, M. Nieddu, C. Antonio Caria, V. Sogos, M. Vallet-Regì, M. Monduzzi, A. Salis, "Mesoporous silica nanoparticles functionalized with hyaluronic acid. Effect of the biopolymer chain length on cell internalization," Colloids and Surfaces B: Biointerfaces 168: (2018) 50-59. [16] B. Katharina, C. Martina Stürzel, J. Biskupek, U. Kaiser, F. Kirchhoff, M. Lindén, "Comparison of different cytotoxicity assays for in vitro evaluation of mesoporous silica nanoparticles," Toxicology in Vitro 52: (2018) 214-221. [17] A. Calvo Tatiana, P. Santagapita, "Physicochemical characterization of alginate beads containing sugars and biopolymers," Journal of quality and reliability engineering 2016 (2016). [18] Levin A. D., A. I. Nagaev, A. Yu Sadagov, "Determination of Number Density of Particles Together with Measurement of Their Sizes by Dynamic Light Scattering," Measurement Techniques 61(8): (2018) 760-76619. [19] Y. Ning-ning, S. Li, G. Li, "Sodium alginate coated mesoporous silica for dual bio-responsive controlled drug delivery," Journal of Drug Delivery Science and Technology 46 (2018): 348-353. [20] O. Jacob, A. Wirsén, Å. Norberg, J. Frie, G. Printz, H. Lagercrantz, G. H. Gudmundsson, B. Agerberth, B. Jonsson, "A novel cysteine-linked antibacterial surface coating significantly inhibits bacterial colonization of nasal silicone prongs in a phase one pre-clinical trial, " Materials Science and Engineering C 93: (2018) 782-789. [21] S. Werner, A. Fink, E. Bohn, "Controlled growth of monodisperse silica spheres in the micron size range," Journal of colloid and interface science 26(1): (1968) 62-69. [22] V. Wan-Ping, B. Beng Lee, A. Idris, A. Islam, B.Ti-Tey, E. Chan, "Production of ultra-high concentration calcium alginate beads with prolonged dissolution profile," RSC Advances 5(46) : (2015) 36687-36695. [23] Ru Feng, Lu Wang, Z. Peng, L. Zhen, Xiaoyu Li, G. Lili, "Development of the pH responsive chitosan-alginate based microgel for encapsulation of Jughans regia L. polyphenols under simulated gastrointestinal digestion in vitro," Carbohydrate Polymers 250: (2020) 116917. [24] D. Annalnsa, B. A.Anna, L. Gaetano, G. Mario, d. Matteo, "Pharmaceutical applications of biocompatible polymer blends containing sodium alginate," Advances in Polymer Technology 31(3) : (2012) 219-230. [25] H. Yan, D. Xiaoying, K. Lei, Z. Shangwen, Z. Dan, C. Han, X. Xincai, "Polysaccharides/mesoporous silica nanoparticles hybrid composite hydrogel beads for sustained drug delivery," Journal of Materials Science, 52(6): (2017) 3095-3109. [26] M. Tanja, C. Marzadori, D. Montecchio, C. Gessa, "Characterisation of Ca–and Al–pectate gels by thermal analysis and FT-IR spectroscopy," Carbohydrate Research 340(16): (2005) 2510-2519. [27] S. Olav, G. Skja., "Alginate as immobilization matrix for cells," Trends in biotechnology 8: (1990) 71-78. [28] C. Bingchao, D. Li, Q. Huo, Q. Zhao, Q. Lan, M. Cui, W. Pan, X. Yang, "Two kinds of ketoprofen enteric gel beads (CA and CS-SA) using biopolymer alginate," asian journal of pharmaceutical sciences 13(2): (2018) 120-130. [29] G. Reddy S., A. Saxena Pandit, "Effect of curing agent on sodium alginate blends using barium chloride as crosslinking agent and study of swelling, thermal, and morphological properties," International Journal of Polymeric Materials and Polymeric Biomaterials 62(14): (2013) 743-748. [30] B. Sara, "Essential oils: their antibacterial properties and potential applications in foods—a review," International journal of food microbiology 94 (3): (2004) 223-253. [31] J. Oju, Kang, K. Sun-Woong, L. Hee-Won, C. Ji Hyung, K. Byung-Soo, "Long-term and zero-order release of basic fibroblast growth factor from heparin-conjugated poly (L-lactide-co-glycolide) nanospheres and fibrin gel," Biomaterials 27(8): (2006) 1598-1607.
Statistics Article View: 301 PDF Download: 274