Effect of Addition Vitamin C and Zinc Chloride in Vitrification Medium on Viability, in vitro Maturation and Ultrastructure Changes of Vitrified Immature Bovine Oocytes | ||
| Journal of Animal and Poultry Production | ||
| Article 2, Volume 11, Issue 11, November 2020, Pages 441-447 PDF (1.32 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jappmu.2020.130060 | ||
| Authors | ||
| Hoda A. M. Yassen* 1; K. A. A. Attia2; Sh. M. Shamiah3; M. N. El-Arian1; M. A. El-Harairy1 | ||
| 1Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt. | ||
| 2Evaluation of Natural Resources Department, Environmental Studies and Research Institute, University of El Sadat City, Egypt. Clinical Nutrition Department, College of Applied Medical Sciences, Jazan University, K.S.A. | ||
| 3Animal Production Research Institute, Agricultural Research Center, Ministry of Agriculture, Doki, Giza, Egypt. | ||
| Abstract | ||
| This study aimed to evaluate the impact of adding vitamin C and zinc chloride to vitrification medium on viability in vitro maturation and ultrastructure changes of vitrified immature Baladi cow oocytes. Compact cumulus oocytes (COCs) (n=1370) were obtained from slaughtered bovine ovaries. Then the morphology of oocytes was examined using a stereomicroscope. Staining with trypan, the in vitro maturation and ultrastructural changes were studied. The results revealed significant (P<0.05) increase of total and normal survival rate of bovine oocytes vitrified with zinc chloride (90.28 and 81.11%) than in vitamin C media (82.5 and 65.65%) or control medium (74.44 and 54.72%). Recovery rate of abnormal bovine oocytes showed significantly an opposite trend (9.17 vs. 16.94 and 19.72%). Proportion of oocytes with viable cytoplasm and viable cumulus (VOVC) was increased significantly (93.75%, P<0.05) in fresh (control) than in medium supplemented with vitamin C and untreated medium (74.55 and 68.63%), respectively. There were non-significant differences among zinc chloride, control and vitamin C media in oocytes with vaible cytoplasm and unvaiable cumulus (VOUC). Supplementation of the vitrification medium with zinc chloride and vitamin C significantly (P<0.05) improved maturation rates (MII) of recovered cumulus oocyte complexes (COCs) than medium without supplementation. The percentage of ultrastructural alterations in most organelles bovine oocytes significantly (P<0.05) increased in oocytes vitrified without supplementation followed by vitamin C, then zinc chloride medium. Conclusion, supplementation of vitamin C or zinc chloride to the vitrification medium improved survival rate, morphologically and ultrastructural, as well as maturation rate of bovine oocytes | ||
| Keywords | ||
| Antioxidants; vitrification; bovine oocyts; ultrastructure | ||
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