Microrna 192 Gene Expression in Type II Diabetic Nephropathy | ||||
| The Egyptian Journal of Hospital Medicine | ||||
| Article 10, Volume 68, Issue 1, July 2017, Page 885-893 PDF (716.73 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.12816/0038187 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Atef A. El-Monem1; Mohamed H. Mahfouz1; Mona A. Mohamed2; Heba Gamal Abd El-Aziz3; Nora Hussien  3
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| 1National institute of diabetes and endocrinology (NIDE) | ||||
| 2Biochemistry Department, Faculty of science (Girls), Al-Azhar University | ||||
| 3Biochemistry Departments, Faculty of Pharmacy (Girls), Al-Azhar University | ||||
| Abstract | ||||
| Background: Diabetic nephropathy (DN) is the common cause of kidney failure in patients with diabetes mellitus. MicroRNAs (miRNAs) are short non-coding RNAs of about 22 nucleotides which recently have been shown to play vital roles in mammalian gene expression. Aim of the study: was to investigate the role of miRNA-192 in the pathogenesis of diabetic nephropathy and disease progression. Patients and Method: Sixty five patients with uncontrolled diabetes mellitus, they were subdivided into; thirty nine patients with normoalbuminuria (<20mg/L); their ages ranged between 48-67 years and the onset of disease between 1-5 years; twenty six patients with microalbuminuria (20-200 mg/L), their ages ranged between 47-66 years and the onset of disease between 5-15 years, in addition to twelve apparently healthy individuals as control; their ages ranged between 51-67 years. Serum Transforming growth factor beta (TGF-β), Interleukin 18 (IL-18) were determined using ELISA technique, the expression level of miRNA-192 in whole blood using (RT-PCR) was determined, other biochemical parameters as fasting plasma glucose (FPG), glycated haemoglobin (HbA1c), lipid profile and creatinine were estimated using commercial available kits. Patients were given written contest. Results: The level of miRNA-192 expressions was significantly lower in microalbuminuria group when compared to normoalbuminuria group. Serum level of IL-18 and TGF-β were significantly higher in both patient groups when compared to control group and their levels were significantly higher in microalbuminuria group than normoalbuminuria group. Conclusion: Together with TGF-β1 and IL-18, miRNA- 192 may not only be used as molecular biomarker in diabetic microvascular complications but also as early marker of alterations in specific biological processes in the kidney. | ||||
| Keywords | ||||
| diabetic nephropathy; miRNA-192; IL-18; TGF-β | ||||
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