Specific IgE Immunoassay for House Dust Mites by Using Multiple Allergen Simultaneous Test (MAST) in Asthmatic Children | ||||
| The Egyptian Journal of Hospital Medicine | ||||
| Article 19, Volume 68, Issue 1, July 2017, Page 967-973 PDF (434.78 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.12816/0038196 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Mohammed Youssef Saad1; Hany Abd El-Hady El-Khaleegy2 | ||||
| 1Department of Parasitology, Faculty of Medicine, Al-Azhar University, New Damietta, Egypt | ||||
| 2Department of Pediatrics, Faculty of Medicine, Al-Azhar University, New Damietta, Egypt | ||||
| Abstract | ||||
| Background: house dust mites are considered as a major cause of bronchial asthma in young children. Inhalation of dust mite allergens by hypersensitive individuals can result in acute attacks of bronchial asthma. Multiple allergen simultaneous test (MAST) for the detection of specific IgE of different allergens is one of the important diagnostic tools for bronchial asthma. Patients and Methods: the study group consisted of forty children suffering from bronchial asthma selected from the outpatient clinic of Pediatrics, Faculty of Medicine, Al-Azhar University, New Damietta, Egypt as well as another normal forty healthy volunteer children were selected as controls. Total IgE immunoassay and quantitative measurement of allergen specific IgE (Biocheck GmbH, Germany) were estimated for all of them. Results: it was found that, out of 27 positive IgE subjects, 20 were positive by poly-check test providing sensitivity of 74.1%; and out of 53 negative IgE subjects, 50 were negative, providing specificity of 94.3%. Conclusion: Poly-check (MAST), in vitro tests can be used in screening for allergens in asthmatic children with high specificity. | ||||
| Keywords | ||||
| house dust mites; bronchial asthma; allergy; specific IgE; multiple allergen simultaneous test (MAST) | ||||
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