EVALUATION OF IMMUNOCHROMATOGRAPHIC ASSAY FOR DIAGNOSIS OF CRYPTOSPORIDIOSIS
MOHAMED, S., EL-HADY, H., AHMED, A. (2020). EVALUATION OF IMMUNOCHROMATOGRAPHIC ASSAY FOR DIAGNOSIS OF CRYPTOSPORIDIOSIS. EKB Journal Management System, 50(3), 477-482. doi: 10.21608/jesp.2020.131060
SHIMAA REFAEY MOHAMED; HANAA AHMED EL-HADY; AMAL MOSTAFA AHMED. "EVALUATION OF IMMUNOCHROMATOGRAPHIC ASSAY FOR DIAGNOSIS OF CRYPTOSPORIDIOSIS". EKB Journal Management System, 50, 3, 2020, 477-482. doi: 10.21608/jesp.2020.131060
MOHAMED, S., EL-HADY, H., AHMED, A. (2020). 'EVALUATION OF IMMUNOCHROMATOGRAPHIC ASSAY FOR DIAGNOSIS OF CRYPTOSPORIDIOSIS', EKB Journal Management System, 50(3), pp. 477-482. doi: 10.21608/jesp.2020.131060
MOHAMED, S., EL-HADY, H., AHMED, A. EVALUATION OF IMMUNOCHROMATOGRAPHIC ASSAY FOR DIAGNOSIS OF CRYPTOSPORIDIOSIS. EKB Journal Management System, 2020; 50(3): 477-482. doi: 10.21608/jesp.2020.131060

EVALUATION OF IMMUNOCHROMATOGRAPHIC ASSAY FOR DIAGNOSIS OF CRYPTOSPORIDIOSIS

Journal of the Egyptian Society of Parasitology
Article 2, Volume 50, Issue 3, December 2020, Page 477-482  XML PDF (677.55 K)
Document Type: Original Article
DOI: 10.21608/jesp.2020.131060
View on SCiNiTO View on SCiNiTO
Authors
SHIMAA REFAEY MOHAMED1; HANAA AHMED EL-HADY1; AMAL MOSTAFA AHMED2
1Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt
2Department of Medical Parasitology, Faculty of Medicine, Sohag University, Egypt.
Abstract
Cryptosporidium is recognized globally as a major etiology of persistent and chronic diarrhea
especially in immunocompromised patients and children with significant morbidity and mortality
rates. Moreover, it is one of the most important pathogens causing waterborne outbreaks.
Hence, rapid diagnosis is crucial. In this study, we tried to determine the diagnostic performance
of Rida®Quick Cryptosporidium cassettes versus microscopy of the modified acid-fast
stained smear. Forty fecal samples were collected from immunocompromised patients complaining
of chronic diarrhea. All samples were examined by Rida®Quick Cryptosporidium cassettes
for copro-antigen detection, and microscopically using concentration techniques and
modified acid-fast staining. Cryptosporidium was detected in (45%) of immunocompromised
patients. The sensitivity, specificity, PPV, and NPV of Rida®Quick Cryptosporidium cassettes
were 88.9%, 95.5%, 94.1% & 91.3% respectively with an accuracy of 92.5%. There was no
cross-reactivity with other intestinal parasites. Rida®Quick Cryptosporidium cassettes provide
adequate sensitivity and specificity and give rapid results. Our results recommend its use as an
alternative test in certain situations especially when screening large populations is needed urgently
in a short time, as in outbreaks and epidemiological surveys.
Keywords
Acid-fast staining; Cryptosporidium; Rapid diagnosis; Rida®quick
Statistics
Article View: 173
PDF Download: 238