ULTRASTRUCTURAL AND IMMUNOHISTOCHEMICAL ALTERATIONS IN HEMOCYTES AND FAT BODY OF THE FLESH FLY (SARCOPHAGA ARGYROSTOMA) LARVAE UPON CANCER CELL LINE INOCULATION | ||||
Journal of the Egyptian Society of Parasitology | ||||
Article 10, Volume 50, Issue 3, December 2020, Page 547-556 PDF (2.28 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jesp.2020.131085 | ||||
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Authors | ||||
LINA A. ABOU EL-KHASHAB1; SHAYMAA H. MAHMOUD2; WALAA A. MOSELHY1; ABDELBASET B. ZAYED1; MOHAMED S. SALAMA3 | ||||
1Department of Zoology and Entomology | ||||
2Faculty of Science, Al-Azhar University (Girls branch), Nasr City, Cairo, Department of Zoology | ||||
3Faculty of Science, Menoufia University, Menoufia, and Department of Zoology | ||||
Abstract | ||||
In this study, hemocytes and fat body changes were investigated after activation the immune response of the third instar larvae of Sarcophaga argyrostoma by injecting the larvae with MDA-MB-231cancer cell lines. To investigate the changes in hemolymph and fat body tissue, light and transmission electron microscopy (TEM) were used. Apoptosis demonstration in the larval flesh fly after injection was immunohistochemically tracked using a marker of caspase initiator activity; namely caspase-3 antibody.The results showed remarkable changes in morphology, viability and the spreading ability of immunocompetent hemocytes and fat body cells between normal and immunized S. argyrostoma larvae. In hemocytes, multicellular aggregates were formed to entrap invading cancer cells, in addition to membrane blebbing, cytoplasm vacuolization, cell and organelle swelling, and chromatin condensation. Meanwhile, in fat body cells of injected larvae, depletion of lipid droplets, changes in the appearance of protein granules and degrade of basement membrane overlying the fat body were observed. These features are typical for apoptotic and autophagal cell death which were confirmed by detection of apoptotic fat body cells after cancer cell injection using activated caspase-3 immunostaining. | ||||
Keywords | ||||
Fat body cells; Hemocytes; Sarcophaga argyrostoma; Cancer cell; Caspase 3; Apoptosis; Ultrastructure; Immunohistochemistry; TEM | ||||
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