PROTOSCOLICES ANTIGENS FROM HYDATID CYST OF HUMAN AND ANIMAL ORIGIN FOR DIAGNOSIS OF CYSTIC ECHINOCOCCOSIS
RAMADAN, R., KHALIFA, M., KAMEL, N., HAMZA, D., ABDEL-WAHAB, A., EL-BAHY, M. (2020). PROTOSCOLICES ANTIGENS FROM HYDATID CYST OF HUMAN AND ANIMAL ORIGIN FOR DIAGNOSIS OF CYSTIC ECHINOCOCCOSIS. EKB Journal Management System, 50(3), 656-663. doi: 10.21608/jesp.2020.131102
REEM M. RAMADAN; MARWA M. KHALIFA; NANCY O. KAMEL; DALIA A. HAMZA; AZZA M. ABDEL-WAHAB; MOHAMED, M. EL-BAHY. "PROTOSCOLICES ANTIGENS FROM HYDATID CYST OF HUMAN AND ANIMAL ORIGIN FOR DIAGNOSIS OF CYSTIC ECHINOCOCCOSIS". EKB Journal Management System, 50, 3, 2020, 656-663. doi: 10.21608/jesp.2020.131102
RAMADAN, R., KHALIFA, M., KAMEL, N., HAMZA, D., ABDEL-WAHAB, A., EL-BAHY, M. (2020). 'PROTOSCOLICES ANTIGENS FROM HYDATID CYST OF HUMAN AND ANIMAL ORIGIN FOR DIAGNOSIS OF CYSTIC ECHINOCOCCOSIS', EKB Journal Management System, 50(3), pp. 656-663. doi: 10.21608/jesp.2020.131102
RAMADAN, R., KHALIFA, M., KAMEL, N., HAMZA, D., ABDEL-WAHAB, A., EL-BAHY, M. PROTOSCOLICES ANTIGENS FROM HYDATID CYST OF HUMAN AND ANIMAL ORIGIN FOR DIAGNOSIS OF CYSTIC ECHINOCOCCOSIS. EKB Journal Management System, 2020; 50(3): 656-663. doi: 10.21608/jesp.2020.131102

PROTOSCOLICES ANTIGENS FROM HYDATID CYST OF HUMAN AND ANIMAL ORIGIN FOR DIAGNOSIS OF CYSTIC ECHINOCOCCOSIS

Journal of the Egyptian Society of Parasitology
Article 23, Volume 50, Issue 3, December 2020, Page 656-663  XML PDF (870.26 K)
Document Type: Original Article
DOI: 10.21608/jesp.2020.131102
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Authors
REEM M. RAMADAN1; MARWA M. KHALIFA1; NANCY O. KAMEL2; DALIA A. HAMZA3; AZZA M. ABDEL-WAHAB1; MOHAMED, M. EL-BAHY1
1Department of Parasitology, Faculty of Veterinary Medicine, Cairo University
2Department of Parasitology, Faculty of Medicine, October 6 University
36th October City, and Department of Zoonoses
Abstract
Hydatid cyst (HC) protoscolices somatic antigens (HCPsS-Ag) derived from the HC extracted
from patients, sheep; equine and HC antigen purchased with ready-made diagnostic kits were
evaluated in diagnosis of zoonotic hydatidosis. The sensitivity and specificity of these antigens
in capturing anti-HC-IgG-antibodies (anti-HC-Abs) were measured by ELISA in infected hosts’
sera. Sensitivity of HCPsS-Ag extracted from HC of human origin to diagnose anti-HC-Abs in
naturally patients was 90.0%. The sensitivity of HCPsS-Ag extracted from sheep, equine and
ready-made diagnostic kits (R.M.K.) were (71.4%; 57.1% & 91.9%) respectively. The median
ELISA OD values of positive HC patient samples versus HCPsS-Ag of sheep and equine was
significant (P≤0.05) lower than screened same sera versus HCPsS-Ag of human origin. For
agreement analysis, Kappa values did show significant difference between human sera versus
HC antigen extracted from human or sheep (K= 0.385, P=0.168). Receiver Operating Characteristics
(ROC) analysis showed accepted human diagnosis by using human or sheep originated
antigens. HCPsS-Ag of human origin showed low cross-reaction with Schistosoma mansoni and
Hepatitis C virus, than those of animal origin.
Keywords
Hydatidosis; Human; Sheep; Equine; Protoscolices; ELISA
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