POTENTIAL IMMUNOMODULATORY EFFECT OF SINGLE AND COMBINED THERAPIES AGAINST CRYPTOSPORIDIUM INFECTION IN IMMUNOSUPPRESSED MOUSE MODEL | ||||
Journal of the Egyptian Society of Parasitology | ||||
Article 25, Volume 50, Issue 3, December 2020, Page 673-682 PDF (1.16 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jesp.2020.131111 | ||||
View on SCiNiTO | ||||
Authors | ||||
MENNAT-ELRAHMAN A. FAHMY1; AMANY A. ABDELAAL2; SOAD I. HASSAN1; MAISA A. SHALABY1; MOUSA A.M. ISMAIL2; RASHA A. KHAIRY3; HANAA O. FADL2 | ||||
1Department of Parasitology | ||||
2Department of Medical Parasitology | ||||
3Department of Pathology | ||||
Abstract | ||||
Cryptosporidium species are related to protozoan parasites that infect the gastrointestinal tract of a wide variety of animals, including humans. The host immune status is crucial for determining the susceptibility to infection, the outcome and the severity of the disease. Nitazoxanide® is the only FDA approved drug to treat such opportunistic infection, yet demonstrates limited and immune-dependent efficacy. The present work studied the effect of combination therapy in treating cryptosporidiosis, plus exploring the possible modulating effect on the local adaptive immune response in experimental dexamethasone immunocompromized mice model. The results showed that combination of selenium supplementation with ivermectin® or nitazoxanide improved the oocysts reduction percentage compared to groups receiving single anti-parasitic therapeutic drugs alone. Also, combination of ivermectin with nitazoxanide gave the best oocysts reduction rates with the lowest score of ileitis severity. But, local expression of CD4 T cells was down-regulated and unlikely could not be elevated enough even after all treatment types. CD8 was up regulated in all treated groups as compared with non-treated control group, indicating its possible role in reducing the serious threats of such opportunistic infection. | ||||
Keywords | ||||
Cryptosporidium; single therapy; combined therapies; CD4; CD8 | ||||
Statistics Article View: 191 PDF Download: 425 |
||||