Evaluation of in vitro bactericidal activity of human serum against Salmonella typhi in relation to serogroups
Aliyu, A., Ismaila, A., Haruna, M., Lambu, Z., Shehu, A., Sadisu, F. (2022). Evaluation of in vitro bactericidal activity of human serum against Salmonella typhi in relation to serogroups. EKB Journal Management System, 3(1), 209-216. doi: 10.21608/mid.2020.52481.1092
Ado Aliyu; Ahmed Ismaila; Musa Haruna; Zakari Lambu; Adamu Shehu; Faruk Sadisu. "Evaluation of in vitro bactericidal activity of human serum against Salmonella typhi in relation to serogroups". EKB Journal Management System, 3, 1, 2022, 209-216. doi: 10.21608/mid.2020.52481.1092
Aliyu, A., Ismaila, A., Haruna, M., Lambu, Z., Shehu, A., Sadisu, F. (2022). 'Evaluation of in vitro bactericidal activity of human serum against Salmonella typhi in relation to serogroups', EKB Journal Management System, 3(1), pp. 209-216. doi: 10.21608/mid.2020.52481.1092
Aliyu, A., Ismaila, A., Haruna, M., Lambu, Z., Shehu, A., Sadisu, F. Evaluation of in vitro bactericidal activity of human serum against Salmonella typhi in relation to serogroups. EKB Journal Management System, 2022; 3(1): 209-216. doi: 10.21608/mid.2020.52481.1092

Evaluation of in vitro bactericidal activity of human serum against Salmonella typhi in relation to serogroups

Microbes and Infectious Diseases
Article 24, Volume 3, Issue 1, February 2022, Page 209-216  XML PDF (403.62 K)
Document Type: Original Article
DOI: 10.21608/mid.2020.52481.1092
View on SCiNiTO View on SCiNiTO
Authors
Ado Aliyu email orcid 1; Ahmed Ismaila1; Musa Haruna2; Zakari Lambu1; Adamu Shehu1; Faruk Sadisu1
1Department of Microbiology, Kano University of Science and Technology, Wudil, Nigeria.
2Department of Biological Science, Kano University of Science and Technology, Wudil, Nigeria.
Abstract
Background: Series of more than 35 proteins facilitated a major role in fighting the foreign invaders in human body and other warm blooded animals, those named complement system. Methods: Total of 147 human serum were collected from asymptomatic volunteers by venipuncture, their sero groups were determined by reverse blood grouping method. Strain of Salmonella typhi (S. Typhi) were collected from Wudil general hospital and identified molecularly at center of biotechnological research of Bayero University, Kano. Serum bactericidal assay was done at microbiology laboratory of Wudil general hospital. Data obtained was subjected to two way ANOVA and considered significant at p < span style="text-decoration: underline;"><0.05.  Results:  Complement dependent pathways shows a more than 50% kills, in which both A, AB, B and O kill percent were 74.98%, 67.87%, 78.53% and 78.60% respectively, however, no statistical significant difference. While the non-complement dependent pathways revealed kills percentage of 59.27%, 57.86%, 59.21% and 58.60% for A, AB, B and O sero groups, also no significant statistical difference. Moreover, in comparing the complement and non-complement dependent pathways, data analysis shows a significant difference with p values of 0.001, 0.003, 0.006 and 0.0008 for A, AB, B and O sero groups respectively. Conclusion: We concluded that, ABO sero-groups and complement system plays an important role as infection determinants, where AB sero-group have more chances to S. Typhi infection than other sero-groupings. This study suggests that the effect of many host genetic parameters on treatment of microorganisms needs to be further studied.
Keywords
Human serum; Complement; Salmonella Typhi
Statistics
Article View: 430
PDF Download: 347