Phenolic Profiling of Hyphaene thebaica by LC-ESI-Mass: Iron Nanoparticles Significance and Cytotoxic Activity
Hussein, S., Elmesallamy, A., Seleim, M. (2021). Phenolic Profiling of Hyphaene thebaica by LC-ESI-Mass: Iron Nanoparticles Significance and Cytotoxic Activity. EKB Journal Management System, 64(4), 1679-1686. doi: 10.21608/ejchem.2020.53478.3109
Sahar Awad Allah Hussein; Amani Elmesallamy; Mai Seleim. "Phenolic Profiling of Hyphaene thebaica by LC-ESI-Mass: Iron Nanoparticles Significance and Cytotoxic Activity". EKB Journal Management System, 64, 4, 2021, 1679-1686. doi: 10.21608/ejchem.2020.53478.3109
Hussein, S., Elmesallamy, A., Seleim, M. (2021). 'Phenolic Profiling of Hyphaene thebaica by LC-ESI-Mass: Iron Nanoparticles Significance and Cytotoxic Activity', EKB Journal Management System, 64(4), pp. 1679-1686. doi: 10.21608/ejchem.2020.53478.3109
Hussein, S., Elmesallamy, A., Seleim, M. Phenolic Profiling of Hyphaene thebaica by LC-ESI-Mass: Iron Nanoparticles Significance and Cytotoxic Activity. EKB Journal Management System, 2021; 64(4): 1679-1686. doi: 10.21608/ejchem.2020.53478.3109

Phenolic Profiling of Hyphaene thebaica by LC-ESI-Mass: Iron Nanoparticles Significance and Cytotoxic Activity

Egyptian Journal of Chemistry
Article 3, Volume 64, Issue 4, April 2021, Page 1679-1686  XML PDF (586.4 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2020.53478.3109
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Authors
Sahar Awad Allah Hussein email orcid 1; Amani Elmesallamyorcid 2; Mai Seleim2
1Head of department of Phytochemistry and Plant Systematics,Division of Pharmaceutical Industries,National Research Center, El Buhouth st., Dokki Dokki, Cairo, Egypt
2Chemistry Department, Faculty of Science, Zagazig University, Egypt.
Abstract
Phenolic profiling of edible parts Hyphaene thebaica ( Doum palm) are identified using LC-ESI-MS, the Overall polyphenolic constituents demonstrated by means of LC-ESI-Mass profiling . Twenty three isolated compounds were identified as ; caffeic acid, protocatechuic acid, rhamnetin, catechin, quercitrin, vanillic acid , kaempferol 3-O-acetyleglucoside , cinnamic acid, apigenin-7-O-glucose , intricatin 3-O-htyrosol, luteolin, quercetin ,naringenin , kaempferol ,vanillic acid 4-β-D-glycoside coumaric acid, ferulic acid, luteolin-6-arbinose-8-glucose, p-coumaroyl malic acid eriocitrin, apigenin and hesperetin
Iron nanoparticles (FeNps ) of H. thebaica fruit EtOAc extract was freshly prepared and characterized with Dynamic Light Scattering (DLS) with particles size 150.7 nm. The anti-proliferation activity of the crude extract of EtOAc and the synthesized Fe Nps was evaluated using MTT assay on human colon (Caco-2) and liver (HepG2) cancer cell lines.
The results declared that half maximal inhibitory concentration (IC50) of EtOAc extract on colon (IC50 35.4 µg/ml) and on liver cancer cell lines (IC50 72.02 µg/ml) while nanoparticles portion of EtOAc was found more pronounced on colon cancer cell lines (IC50 19.44 µg/ml) and on liver (IC50 15.5 µg/ml). So, the Fe Nps of EtOAc of H.thebaica fruit extract with particles size 150.7 nm is more effective as antitumor than the crude EtOAc extract.
Keywords
Hyphaene thebaica; LC-ESI-MS; polyphenolic constituents; Iron Nanoparticles Fe Nps; Antitumor activity
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