First Record of an Isolate (Pathotype) of Puccinia striiformis f. sp. hordei the Causative of Stripe Rust on Barley in Egypt | ||||
Egyptian Journal of Phytopathology | ||||
Article 18, Volume 47, Issue 1, July 2019, Page 367-369 PDF (978.98 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejp.2019.132189 | ||||
View on SCiNiTO | ||||
Authors | ||||
Badawy R. Mohdly 1; Ashraf E. Khalil1; Khairy A. Amer2 | ||||
1Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt. | ||||
2Field Crops Research Institute, Agricultural Research Center, Giza, Egypt. | ||||
Abstract | ||||
During 2018/2019 growing season, symptoms of stripe rust (yellow rust), caused by Puccinia striiformis f. sp. hordei, on barley leaves (Hordeum vulgare L.) were observed, specifically on (LIGNEE527/GERBEL/3/BOY/B*2/SURB//CI1225.2D/4/M104) and (Kv//Alger/Ceres.362/1/1/3/Arr/Esp//Alger/Ceres.362/1/1/4/CamB1//CI08887/CI05761/3/Kataja//Esp/1808/4L/5/Harmal02ArabiAbiad*2/4/Soufara02/3/R1M508/Por//WI2269Nawair1) lines, grown in El-Alameen region - Marsa Matrouh governorate (Fig., 1). Isolation of the causative was carried out from single pustules, multiplied and the obtained urediospores were used to carry out pathogenicity test on the same lines. Typical symptoms caused by Puccinia striiformis f. sp. hordei were obtained after 14 days and found to be similar to those previously collected from infected barley samples. Definition of pathogen Pathotype of P. striiformis f. sp. hordei using the molecular biology method (RAPD) DNA showed 99% similarity with Pathotype of Puccinia striiformis f. sp. hordei. On the basis of the Blast Fungi, Puccinia striiformis f. sp. hordei appeared high similarity with Pathotype as shown in (Fig., 2). This Isolate was recorded in the NCBI/gene bank under the number MN598662. This is the first record of the pathogen Pathotype of yellow rust in Egypt. | ||||
Keywords | ||||
barley; Hordeum vulgare; stripe rust; yellow rust; Puccinia striiformis f. sp. hordei | ||||
Statistics Article View: 210 PDF Download: 195 |
||||