Molecular Identification of Grey Mullet species in the Mediterranean Sea of Egypt
Abou-Gabal, A., Abbas, E., Ali, H., El-Baramawi, N., Khaled, A., El Deeb, S. (2018). Molecular Identification of Grey Mullet species in the Mediterranean Sea of Egypt. EKB Journal Management System, 22(4), 125-139. doi: 10.21608/ejabf.2018.13263
Ashgan A. A. Abou-Gabal; Eman M. Abbas; Hala Mahmoud Mohamed Ali; Nagy El-Baramawi; Asmaa A. Khaled; Safaa I. El Deeb. "Molecular Identification of Grey Mullet species in the Mediterranean Sea of Egypt". EKB Journal Management System, 22, 4, 2018, 125-139. doi: 10.21608/ejabf.2018.13263
Abou-Gabal, A., Abbas, E., Ali, H., El-Baramawi, N., Khaled, A., El Deeb, S. (2018). 'Molecular Identification of Grey Mullet species in the Mediterranean Sea of Egypt', EKB Journal Management System, 22(4), pp. 125-139. doi: 10.21608/ejabf.2018.13263
Abou-Gabal, A., Abbas, E., Ali, H., El-Baramawi, N., Khaled, A., El Deeb, S. Molecular Identification of Grey Mullet species in the Mediterranean Sea of Egypt. EKB Journal Management System, 2018; 22(4): 125-139. doi: 10.21608/ejabf.2018.13263

Molecular Identification of Grey Mullet species in the Mediterranean Sea of Egypt

Egyptian Journal of Aquatic Biology and Fisheries
Article 9, Volume 22, Issue 4, September 2018, Page 125-139  XML PDF (9.12 MB)
Document Type: Original Article
DOI: 10.21608/ejabf.2018.13263
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Authors
Ashgan A. A. Abou-Gabal1; Eman M. Abbasorcid 2; Hala Mahmoud Mohamed Ali3; Nagy El-Baramawi1; Asmaa A. Khaled1; Safaa I. El Deeb2
1Faculty of Agriculture, Saba Basha, Alexandria University, Alexandria, Egypt
2Genetic and Genetic Engineering Department, National Institute of Oceanography and Fisheries, Alexandria, Egypt
3Genetics, Faculty of Agriculture, Alexandria University.
Abstract
Mullet fish provide a valuable role in aquaculture to meet the rising demands for fish production in Egypt. The accurate estimate of species limits is a key factor in improving mullet fish for the application in evolution, conservation and management of the genetic programs. Four species of mullet fish, “Mugil cephalus, Liza ramada, Liza aurata, and Liza saline” were investigated by RAPD analysis to assess the genetic structure. Mullet fish were collected from three locations in the Mediterranean Sea (Al Maadyeah – Al Manzala – Bardawil). RAPD assay was performed using 10 decamers random primers; all the ten primers were succeeded in producing distinct bands.The obtained results revealed that the number of reproducible bands analyzed for ten primers for Al Maadyeah fishes was 181 bands in all four species, but in Bardawil and Manzala were 224 and 229 respectively. In each interspecies Mullet variation, the polymorphic amplified bands were 78.12% from it (20.98% M. cephalus 5.35% L. aurata, and 16.07 % Liza ramadaunique bands) in Al Manzala, and 73.79% polymorphic from it (20.96% M. cephalus, 4.36% L. aurata and 12.2% L. ramada unique bands) in Bardawil and 66.85% polymorphic bands for Al Maadyeah from it (11.6% M. cephalus 8.2% L. aurata, 1.1% for L. ramada and L. salineunique Bands). Nei’s genetic distance between (0.503 – 0.719) in Al Manzala, (0.263 – 0.514) in Al Maadyeah and (0.271 – 0.537) in Bardawil. The RAPD technique can be introduced as an easy tool in the population genetics of marine fishes, providing supplementary information in their genetic stock structure analysis.
Keywords
Mullet fish; Mediterranean Sea; RAPD; PCR; Mugilidae Family; species differentiation; phylogenetic tree
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