Physiological and Molecular Studies on Thermotolerance of Bacillus cereus Isolated from Some Dairy Products and Fast Foods | ||||
| Zagazig Veterinary Journal | ||||
| Article 2, Volume 48, Issue 4, December 2020, Page 354-365 PDF (617.12 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zvjz.2020.35504.1113 | ||||
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| Authors | ||||
Aham Gharib; Marwa Abd El-Hamid; Engy Ayob 
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| Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig, Sharkia Governorate, Egypt | ||||
| Abstract | ||||
| Several members of Bacillus cereus (B. cereus) have been recognized as a significant agent of food and health problems. Thermotoleranc of B. cereus after pre-exposure to temperature shifts or other stress may influence its ability to grow in minimally processed foods. This study was proposed to determine the recovery rate of B. cereus group from dairy products and fast foods. Moreover, the responses of a recovered isolate to different stresses were evaluated to stand upon its thermotolerance against lethal temperature. In total, 200 different samples from milk and milk products (164) and fast food (36) were examined for the presence of B. cereus group. The effects of heat, cold, acid and salt stresses on heat resistant B. cereus were physiologically and molecularly assessed through examining its viability and measuring the gene expression of its heat induced proteins (GroEL and DnaJ) by reverse transcription quantitative polymerase chain reaction (RT-qPCR), respectively. Overall, 35 (17.5%) samples were positive for B. cereus group isolates. The higher B. cereus count (> 104 CFU/mL or g) was found in 7% of milk and fast food samples. Three B. cereus group species (B. cereus, B. thuringiensis and B. pseudomycoides or B. mycoides) were identified. After time intervals of mild heat pre-exposure at 42 °C or exposure to other stresses, B. cereus developed an increased thermotolerance at 50 °C/20 min. This thermotolerance was pronounced after exposure of B. cereus cells to 42°C /1 h and 2.5 % salt / 30°C/ 40 min with a 3.9 CFU log increase compared to those exposed to lethal treatment only. Molecularly, RT-qPCR results revealed up-regulation of dnaJ gene expression in pretreated cells compared to the lethal treated cells only. Overall, these results confirmed the heat resistance occurs in B. cereus during food processing, which results in its survival in the food products. | ||||
| Keywords | ||||
| B. cereus; viability; Thermotolerance; dnaJ gene; B. cereus RT-qPCR | ||||
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