THE EFFECT OF POLYETHYLENE GLYCOL (PEG) ON CALLUSES OF SWEET BASIL (OCIMUM BASILICUM L.)
Osman, A., El-Kadafy, A., Sewedan, E., Moubarak, M., Abdel-Rahman, M. (2020). THE EFFECT OF POLYETHYLENE GLYCOL (PEG) ON CALLUSES OF SWEET BASIL (OCIMUM BASILICUM L.). EKB Journal Management System, 7(4), 447-459. doi: 10.21608/sjfop.2020.134610
Amira Osman; Aida I.R. El-Kadafy; Eman Sewedan; Manee Moubarak; Manal Abdel-Rahman. "THE EFFECT OF POLYETHYLENE GLYCOL (PEG) ON CALLUSES OF SWEET BASIL (OCIMUM BASILICUM L.)". EKB Journal Management System, 7, 4, 2020, 447-459. doi: 10.21608/sjfop.2020.134610
Osman, A., El-Kadafy, A., Sewedan, E., Moubarak, M., Abdel-Rahman, M. (2020). 'THE EFFECT OF POLYETHYLENE GLYCOL (PEG) ON CALLUSES OF SWEET BASIL (OCIMUM BASILICUM L.)', EKB Journal Management System, 7(4), pp. 447-459. doi: 10.21608/sjfop.2020.134610
Osman, A., El-Kadafy, A., Sewedan, E., Moubarak, M., Abdel-Rahman, M. THE EFFECT OF POLYETHYLENE GLYCOL (PEG) ON CALLUSES OF SWEET BASIL (OCIMUM BASILICUM L.). EKB Journal Management System, 2020; 7(4): 447-459. doi: 10.21608/sjfop.2020.134610

THE EFFECT OF POLYETHYLENE GLYCOL (PEG) ON CALLUSES OF SWEET BASIL (OCIMUM BASILICUM L.)

Scientific Journal of Flowers and Ornamental Plants
Article 7, Volume 7, Issue 4, December 2020, Page 447-459  XML PDF (1.04 MB)
Document Type: Original Research Article
DOI: 10.21608/sjfop.2020.134610
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Authors
Amira Osman email orcid 1; Aida I.R. El-Kadafy1; Eman Sewedan1; Manee Moubarak1; Manal Abdel-Rahman2
1Horticulture Department, Agriculture Faculty, Damanhour University, Egypt
2Genetic Engineering and Biotechnology Laboratory, Plant Pathology Department (Genetic Branch), Agriculture Faculty, Damanhour University, Egypt.
Abstract
Ocimum basilicum L. sweet basil is native to India and Iran, an aromatic herb and perennial belong to the Lamiaceae family. This study was done at the Genetic Engineering and Biotechnology Laboratory, Faculty of Agriculture, Damanhour University, Egypt from 2019 to 2020. Developing stress tolerant plants using in vitro selection is an effective method; therefore, the purpose of this study was to detect the best concentration of phytohormones to produce embryogenic callus of Ocimum basilicum L. and study the effect of poly ethylene glycol (PEG) on embryogenic callus of basil.Leaf specimens of basil were cultured on MS medium (Murashige and Skoog) with concentration of auxin and cytokinin.  A1 (2, 4-D at 0.5 mg/l), A2 (2, 4-D at 1.0 mg/l) and A3 (2, 4-D and BAP at 1.0 mg/l and 0.2 mg/l). Embryogenic callus of basil was sub cultured under normal and drought stress media containing different concentration of polyethylene glycol (PEG; M.W 4000) at 2, 5 and 10%. Results showed that medium A3 was the best medium of embryogenic callus induction. Also, the concentration of PEG at 5% was shown maximized callus survivability compared with the other PEG concentrations with no significant effect. This study adds new information on the effect of 5% PEG which showed the highest (positive) survivability of callus. The morphogenic of calluses on 5% PEG were yellowish, friable while the morphogenic of calluses on 10% PEG turned into brown.
Keywords
Ocimum basilicum L; sweet basil embryogenic callus; polyethylene glycol (PEG); in vitro selection; drought stress
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