Evaluation of Nano-Based-ELISA for Serodiagnosis of Human Toxoplasmosis | ||||
| Benha Journal of Applied Sciences | ||||
| Article 24, Volume 5, Issue 2 part (1), February 2020, Page 161-167 PDF (876.2 K) | ||||
| Document Type: Original Research Papers | ||||
| DOI: 10.21608/bjas.2020.135449 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| A.S. Al hamshary1; I.R. Bayoumi2; N.S. Aly1; R.E. Omar1; D.A. Mohammed1; Y.M. Marei3; G. A. Rashed1 | ||||
| 1Parasitology Dept., Faculty of Medicine, Benha Univ., Benha, Egypt | ||||
| 2ImmunoParasitology Dept., Theodor Bilharz Research institute, Imbaba, Giza, Egypt | ||||
| 3Biochemistry and Molecular Biology,Dept., Faculty of Medicine, Benha Univ., Benha, Egypt | ||||
| Abstract | ||||
| Toxoplasma surface antigen 1(SAG1) is a major surface antigen involved in the diagnosis of toxoplasmosis. Although it only accounts for 3-5% of total T. gondii protein, the majority of the antibodies are reactive against SAG1 during infection. SAG1 has potent immunogenicity and immune-reactivity; therefore, it is widely applied in diagnostic techniques. Will assess the symptomatic execution of a novel nano symptomatic elisa test Previously, serodiagnosis for human toxoplasmosis. Generation for list 1 What's more its purification might have been carried accompanied Eventually Tom's perusing preparation about igigi polyclonal antibodies Also its purification, characterization Furthermore conjugation with gold nanoparticles identification of Toxoplasma (SAG1) in the serum by nano-gold sandwich elisa uncovered 89. 2% affectability Also 94%, specificity. Nano-gold elisa method is a delicate test to identification of mankind's toxoplasmosis. | ||||
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