Comparative Study between Croton tiglium Seeds and Moringa oleifera Leaves Extracts, after Incorporating Silver Nanoparticles, on Murine Brains

Aboulthana, Wael M.; Youssef, Ahmed M.; Seif, Mohamed M.; Osman, Noha M.; Sahu, Ram Kumar; Ismael, Mohamed; El-Baz, Hatim A.; Omar, Nagwa I.

doi:10.21608/ejchem.2021.53777.3113

	Comparative Study between Croton tiglium Seeds and Moringa oleifera Leaves Extracts, after Incorporating Silver Nanoparticles, on Murine Brains
Egyptian Journal of Chemistry
Article 6, Volume 64, Issue 4, April 2021, Page 1709-1731 PDF (1.32 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.53777.3113
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Authors
Wael M. Aboulthana ¹; Ahmed M. Youssef ²; Mohamed M. Seif ³; Noha M. Osman⁴; Ram Kumar Sahu ⁵; Mohamed Ismael⁶; Hatim A. El-Baz ¹; Nagwa I. Omar⁷
¹Biochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt (Affiliation ID: 60014618).
²Packaging Materials Department, National Research Centre, 33 Bohouth St., P.O. 12622, Dokki, Giza, Egypt (Affiliation ID: 60014618).
³Toxicology and Food Contaminants Department, Food Industry and Nutrition Division, National Research Center
⁴Cell Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, 33 Bohouth St., P.O. 12622, Dokki, Giza, Egypt (Affiliation ID: 60014618).
⁵University College of Pharmacy, Pt. Deendayal Upadhyay Memorial Health Sciences and Ayush University of Chhattisgarh, Raipur (C.G.), India.
⁶Energy and Environmental Sciences Laboratory, Chemistry Department, Faculty of Science, Sohag University, Egypt.
⁷Biochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt affiliation ID: 60014618.
Abstract
Croton tiglium seeds and Moringa oleifera leaves extracts are rich in phytoconstituents with the antioxidant efficiency which can be enhanced by incorporating silver nanoparticles (Ag-NPs). The present study was designed to compare the effect of C. tiglium seeds and M. oleifera leaves nano-extracts on brain tissues of murine models. During the current study, acetylcholine esterase (ACHE), β-amyloid (Aβ) content and inflammatory markers were measured in brain tissues. Moreover, native protein, lipoprotein and isoenzymes patterns were electrophoretically detected. Also, the interferon-gamma (INF-γ) receptor protein was studied by molecular dynamic simulation to evaluate the significant alterations on brain tissues. It was found that ACHE, Aβ contents and inflammatory markers increased in the C. tiglium nano-extract treated group at a dose of 6.5 ml/kg. Furthermore, it caused qualitative electrophoretic abnormalities represented by lowering similarity index (SI) values. Also, the resides range 119~127 represent the most reactive and flexible site in INF-γ receptor protein. On the other hand, it was shown that no significant differences were induced by silver M. oleifera nano-extract.
Keywords
Croton tiglium Seeds; Moringa oleifera Leaves; Nanotechnology; Electrophoresis; Molecular Dynamic Modeling


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