BIOTECHNOLOGICAL STUDIES ON Solanum viride PLANT: A- IN VITRO PROPAGATION OF Solanum viride PLANT THROUGH TISSUE CULTURE TECHNIQUE. | ||||
| Journal of Plant Production | ||||
| Article 23, Volume 33, Issue 3, March 2008, Page 2133-2148 PDF (946.75 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2008.135766 | ||||
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| Authors | ||||
| H. A. Emara1; I. A. Ibrahim1; M. H. EL-Massry2; A. A. Dahab2 | ||||
| 1Dept. of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, Minufiya University, Egypt. | ||||
| 2Dept. of Medicinal and Aromatic Plants, Horticulture Research Institute. | ||||
| Abstract | ||||
| Leaves of Green nightshade (Solanum viride Solander ex Forst. f.) family: Solanaceae were used as source of explants to start micropropagation. Leaves were successfully sterilized using the treatment of sodium hypochlorite (NaOCl) at 1.0% that showed the highest percentage of survival without any contamination. The highest fresh weight of callus/explant was recorded when the leaf segments were cultured on MS media contained 0.5 mg/l BA and 1.5 mg/l NAA. Interestingly, some treatments recorded direct organogenesis (direct shoots and roots) from the leaf explants. The highest direct shoot formation was recorded with MS media supplemented with 2 mg/l BA alone. However, it was clear that all shoots obtained with the responded treatments were vitrified. Therefore, in a trial to obtain unvitrified shoots as indirect organogenesis through callus formation and differentiation, all indirect obtained shoots were vitrified except some shoots (4.7 % of them) obtained on MS media supplemented with 1.0 mg/l BA alone. These unvitrified shoots were successfully acclimatized in soil mixture of sand and peatmoss (1:2, v: v) that showed the best growth and percentage of survival (95%). | ||||
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